XU Nan. Herbs Combination of Extraction Process for Banxia Baizhu Tianma Tang by Semi-bionic Enzyme Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 16-19.
DOI:
XU Nan. Herbs Combination of Extraction Process for Banxia Baizhu Tianma Tang by Semi-bionic Enzyme Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 16-19. DOI: 10.13422/j.cnki.syfjx.2015130016.
Herbs Combination of Extraction Process for Banxia Baizhu Tianma Tang by Semi-bionic Enzyme Method
Objective: To ascertain extract with relative molecular weight cutoff ≤1 kDa as the final product
then optimize extracting combination of herbs in Banxia Baizhu Tianma Tang with semi-bionic enzyme method. Method: Herbs combination was divided into 15 groups
taking yields of gastrodine
glycyrrhetinic acid
total flavonoids
extract and total area of extract HPLC as indexes
extracting combination of herbs in Banxia Baizhu Tianma Tang with semi-bionic enzyme method was optimized by comprehensive scoring. Result: The best combination of six herbs was as following:mixing extraction of Pinelliae Rhizoma
Gastrodia Rhizoma and Atractylodis Macrocephalae Rhizoma
mixing extraction of Poria
Citri Exocarpium Rubrum and Glycyrrhizae Radix et Rhizoma.Yields of gastrodine
glycyrrhetinic acid
total flavonoids
extract and total area of extract HPLC were 1.552 8 mg·g-1
189.864 2 μg·g-1
6.767 4 mg·g-1
0.238 1 g·g-1
7 566.3. Conclusion: This optimized herbs combination is rational and feasible for providing a reference for extraction process of Banxia Baizhu Tianma Tang.