Optimization of Purification Technology of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 27-30.
DOI:
Optimization of Purification Technology of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 27-30. DOI: 10.13422/j.cnki.syfjx.2015130027.
Optimization of Purification Technology of Total Flavonoids from
Objective: To optimize purification technology of total flavonoids from Hyssopus officinalis by macroporous resin. Method: Adsorption and desorption capacity of total flavonoids was taken as index
11 different types of macroporous resins were screened
static adsorption-elution test were carried out.Single factor test was used to investigate purification technology of total flavonoids from H. officinalis. Result: AB-8 macroporous resin was the most suitable one for purification of total flavonoids in H. officinalis.Optimum purification parameters were as followings:the sample concentration of 2.56 g·L-1
sample flow rate of 4 BV·h-1
diameter-high ratio of resin bed 1: 5
washed impurity with 5 BV of water
elution flow rate of 5 BV·h-1 and discarded eluent
eluted with 9 BV of 70% ethanol at 4 BV·h-1.Purity of total flavonoids reached 65.6%. Conclusion:This purification technology is stable
feasible and suitable for purification of total flavonoids from H. officinalis.