Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode

WANG Feng-yun; JIANG Ying-shi; LAI Xiao-ping

doi:10.13422/j.cnki.syfjx.2015130036

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Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode

更新时间：2024-01-04

- Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 13, Pages: 36-40(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015130036
  CLC： R346;R282.5
- Published：2015
- 稿件说明：
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WANG Feng-yun, JIANG Ying-shi, LAI Xiao-ping. Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 36-40.
DOI：

WANG Feng-yun, JIANG Ying-shi, LAI Xiao-ping. Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 36-40. DOI： 10.13422/j.cnki.syfjx.2015130036.

摘要

目的: 利用DNA条形码鉴定技术

快速、准确地鉴别不同种类及产地的燕窝

为该药材的质量评价提供参考。方法: 提取不同产地及品种燕窝的DNA

对烟酰胺腺嘌呤二核苷酸(NADH)脱氧酶亚单位2(ND2)部分序列进行扩增和测序

利用软件MEGA6.0进行序列比对

分析变异位点

计算Kimura-2参数遗传距离

构建邻接(neighbor-joining

NJ)系统发育树。结果: NJ系统发育树显示31个官燕窝样品均与爪哇金丝燕Aerodramus fuciphagus聚为1支;1个毛燕窝样品与大金丝燕A. maximus聚为1支。但官燕窝中经细胞色素b(Cytb)鉴定生物基原为爪哇金丝燕亚种(淡腰金丝燕A. fuciphagus germani)的8个样品单独聚为1支

支持率82%

并在该段ND2序列表现为2个位点的差异

表明该段ND2序列可鉴别爪哇金丝燕的亚种。结论: ND2条形码可用于快速、准确地鉴别燕窝的生物基原。

Abstract

Objective: To identificate different origins and categories of commodity edible bird's nest (EBN) by DNA barcoding technique fastly and acurrately. Method: Total genomic DNA was isolated from different origins and categories of commodity EBN. Nicotinamide adenine dinucleotide dehydrogenase subunit 2 (ND2) gene sequences were amplified and sequenced.Identification were performed using similar Blast from GenBank

nearest distance and neighbor-joining (NJ) methods. Result: NJ phylogenetic tree showed 31 white or silver EBN samples which were clustered with Aerodramus fuciphagus;a black EBN sample was clustered with A. maximus.However

among white or silver EBN samples

there were 8 samples clustered into a separate branch with support rate of 82%

which were identified by cytochrome b (Cytb) gene as the subspecies (A. fuciphagus germani)

and sequence alignment showed 2 parsimony-informative sites.This result showed that segment of ND2 sequence can identify subspecies of A. fuciphagus. Conclusion: ND2 sequence is efficient barcode for identifying origin species of EBN.

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