Objective: To investigate quality differences and accumulation law of steroidal saponins within Paris polyphylla var. yunnanensis

to provide a basis for quality evaluation and quality germplasm screening of the medicinal material. Method: UPLC was employed to determine contents of polyphyllin Ⅰ

Ⅱ

Ⅵ and Ⅶ from roots

stems and leaves in the same population of P. polyphylla var. yunnanensis.UPLC fingerprint of different parts was established

quality differences of P. polyphylla var. yunnanensis and its accumulation law of steroidal saponins were analyzed. Result: Contents of total saponins from roots and leaves in the same population of P. polyphylla var. yunnanensis were more than 0.6%.There were significant differences about contents of polyphyllin Ⅰ

Ⅱ and Ⅶ

but polyphyllin Ⅵ showed no significant difference.Five common peaks of UPLC fingerprint were selected in roots

stems and leaves

similarity of characteristic peaks were above 0.753. Conclusion: Polyphyllin Ⅰ

Ⅱ

Ⅵ and Ⅶ are synthesized

stored and distributed in the chloroplasts of green plants

then transported down by stems and majorly accumulated in leaves and rhizomes at last

accumulation law of total saponins content in different parts is leaves>roots>stems.There are large differences about contents and species of main active ingredients among roots

stems and leaves of P. polyphylla var. yunnanensis.