ZHU Jiang, CAI Ming-chen, WANG Xin, et al. Quantitation of Triacylglycerols in Cannabis Semen Using Non-aqueous Reversed-phase High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 53-55.
DOI:
ZHU Jiang, CAI Ming-chen, WANG Xin, et al. Quantitation of Triacylglycerols in Cannabis Semen Using Non-aqueous Reversed-phase High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 53-55. DOI: 10.13422/j.cnki.syfjx.2015130053.
Quantitation of Triacylglycerols in Cannabis Semen Using Non-aqueous Reversed-phase High Performance Liquid Chromatography
Objective: To establish a quantitation of triacylglycerols in Cannabis Semen by non-aqueous reversed-phase high-performance liquid chromatography. Method: The chromatography was carried out on Hypersil ODS2 column (4.6 mm×250 mm
5 μm) with acetonitrile-isopropanol(75: 25) as the mobile phase at a flow rate of 1.0 mL·min-1
the detection wavelength was set at 205 nm. Result: The linear rang of linolein was 0.016-0.33 g·L-1 (r=0.999 8). The average recovery was 97.6%
RSD 1.4%. Conclusion: A non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) was established for determination triacylglycerols in Cannabis Semen.