HU Ying-jie. Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 62-65.
DOI:
HU Ying-jie. Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 62-65. DOI: 10.13422/j.cnki.syfjx.2015130062.
Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC
目的: 建立HPLC同时测定小儿清肺化痰口服液中5种成分(盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷、黄芩苷、白花前胡甲素)含量的方法。 方法: 采用ZORBAX SB-C18色谱柱(4.6 mm×250 mm
5 μm)
流动相乙腈-0.1%磷酸水溶液
梯度洗脱
体积流量 0.8 mL·min-1
柱温35 ℃
DAD检测器
检测波长210 nm(盐酸麻黄碱、盐酸伪麻黄碱)
225 nm(苦杏仁苷)
277 nm(黄芩苷)
321 nm(白花前胡甲素)。 结果: 5个成分的峰面积与质量浓度的线性关系良好
加样回收率98.52%~99.93%。 结论: 该方法简便、准确、灵敏
重复性好
可用于测定小儿清肺化痰口服液中5种成分的含量。
Abstract
Objective: To develop a HPLC method for determining five constituents (ephedrine hydro-chlorid
pseudoephedrine hydrochloride
amygdalim
baicalin and praeruptorin A) in Xiaoer Qingfei Huatan oral solution. Method: Five constituents were analyzed by ZORBAX SB-C18(4.6 mm×250 mm
5 μm) with acetoni-trile-water (containing 0.1% phosphonic acid) as mobile phases in gradient elution
0.8 mL·min-1 as flow-rate and 35 ℃ as temperature of column. The detection wavelengths were at 210 nm for ephedrine hydrochloride and pseudoephedrine hydrochloride
225 nm for amygdalim
277 nm for baicalin and 321 nm for praeruptorin A respectively. Result: Five constituents were seperated perfectly and good linear relationships were obtained. The recoveries were between 98.52%-99.93%%. Conclusion: The developed method is simple
specific
accurate and with good reproducibility
which can be used for quality control of Xiaoer Qingfei Huatan oral solution.
Determination of Dphedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Qianbai Biyan Tabelt by CE
Optimization of Extraction Process of Xiaoer Pingchuan Qutan Granule by Orthogonal Design
Effect of Berberine-Baicalin Combination on Fecal Microbiota Transplantation-induced Type 2 Diabetes Mellitus Due to Internal Accumulation of Dampness-heat in Mice from Perspectives of Gut Microbiota and Metabolomics
Baicalin Induces Ferroptosis in Gastric Cancer Cells via p53-mediated SLC7A11 Down-regulation
Investigation of Effect of Polyethylene Glycol 400 on Pharmacokinetics and Anti-inflammatory Effect of Baicalin Based on UPLC-MS/MS and Molecular Docking Techniques
Related Author
DING Jia-jia
LI Zheng-zheng
SONG Fen-yun
YANG De-bin
TONG Yan
MA Zhen-shan
WANG Lin
DONG Mei-hong
Related Institution
Research Center for Differentiation and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Jiangxi University of Chinese Medicine
Henan Academy of Chinese Medicine
The Second Clinical Medical College, Henan University of Chinese Medicine
State Key Laboratory of Functions and Applications of Medicinal Plants,School of Pharmaceutical Sciences,Engineering Center of Microbiology and Biochemical Pharmacy,Experimental Animal Center,Guizhou Medical University
Chengdu University of Traditional Chinese Medicine