Determination of Plasma Concentrations of Seven Constituents in Zishen Qinggan Daiping Formula and Their Pharmacokinetics in Rats

TIAN Chun-yu; BO Hai-mei; LA Xiao-jin; ZHU Liang; CAO Hui-juan; YAN Xin; LI Ji-an

doi:10.13422/j.cnki.syfjx.2015130077

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Determination of Plasma Concentrations of Seven Constituents in Zishen Qinggan Daiping Formula and Their Pharmacokinetics in Rats

更新时间：2024-01-04

- Determination of Plasma Concentrations of Seven Constituents in Zishen Qinggan Daiping Formula and Their Pharmacokinetics in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 13, Pages: 77-81(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015130077
  CLC： R284;R285
- Published：2015
- 稿件说明：
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TIAN Chun-yu, BO Hai-mei, LA Xiao-jin, et al. Determination of Plasma Concentrations of Seven Constituents in Zishen Qinggan Daiping Formula and Their Pharmacokinetics in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 77-81.
DOI：

TIAN Chun-yu, BO Hai-mei, LA Xiao-jin, et al. Determination of Plasma Concentrations of Seven Constituents in Zishen Qinggan Daiping Formula and Their Pharmacokinetics in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 77-81. DOI： 10.13422/j.cnki.syfjx.2015130077.

摘要

目的: 建立UPLC-MS-MS同时测定大鼠血浆中2

4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷

1-脱氧野尻霉素和决明子苷、红镰霉素龙胆二糖苷、橙黄决明素、甲基钝叶决明素、白藜芦醇的分析方法

研究大鼠服用滋肾清肝代平方后的药代动力学特征。方法: 采用Waters Acquity UPLC BEH C18色谱柱

流动相0.1%甲酸水溶液-乙腈梯度洗脱

采用电喷雾电离源

扫描方式为多反应离子监测。结果: 7种成分在大鼠血浆中线性关系良好

提取回收率94.83%~106.58%

日内、日间精密度和准确度良好。7种成分在模型组大鼠中的药时曲线下面积、末端半衰期、达峰时间、清除率、表观分布容积、药峰浓度分别为(326.65±26.66) μg·L-1·h-1

(3.64±1.69) h

0.33 h

(60.56±5.32) L·h-1·kg-1

(325.13±167.18) L·kg-1

(169.25±18.02) μg·L-1。结论: 该方法特异、快速、准确、灵敏

可用于滋肾清肝代平方在大鼠体内的药动学研究。

Abstract

Objective: To establish a UPLC-MS-MS analysical method for simultaneous determination of concentrations of 2

4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside

l-deoxynojirimycin

cassiaside

rubrofusarin gentiobioside

aurantio-obtusin

chryso-obtusin

resveratrol in rat plasma and to study their pharmacokinetic characteristics after taking Zishen Qinggan Daiping formula in rats. Method: Acidified plasma samples were precipitated for protein with methanol.Waters Acquity UPLC BEH C18 column was employed with mobile phase of 0.1% formic acid-water and acetonitrile for gradient elution.Detection was carried out by multiple reaction monitoring (MRM) positive ion mode with ESI ionization source. Result: Seven constituents showed a good linear relationship with extraction recovery ranging between 94.83%-106.58% and a high intra-and inter-day precisions and accuracy.Their pharmacokinetic parameters in model group rats were AUC0-∞ of (326.65±26.66) μg·L-1·h-1

t1/2 of (3.64±1.69) h

Tmax of 0.33 h

CLz/F of (60.56±5.32) L·h-1·kg-1

Vz/F of (325.13±167.18) L·kg-1

Cmax of (169.25±18.02) μg·L-1. Conclusion: This mentioned method is specific

rapid

sensitive and suitable for pharmacokinetic studies of Zishen Qinggan Daiping formula in rats.

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Related Institution

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