Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell

FENG Yan-ying; LIU Hua-gang; LIANG Yan; ZHANG Hua-jun; LIANG Qiao-fang; LI Jun-wei

doi:10.13422/j.cnki.syfjx.2015130095

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Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell

更新时间：2024-01-04

- Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 13, Pages: 95-99(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015130095
  CLC： R96
- Published：2015
- 稿件说明：
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FENG Yan-ying, LIU Hua-gang, LIANG Yan, et al. Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 95-99.
DOI：

FENG Yan-ying, LIU Hua-gang, LIANG Yan, et al. Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 95-99. DOI： 10.13422/j.cnki.syfjx.2015130095.

摘要

目的: 研究氯化两面针碱(nitidine chloride

NC)对人卵巢癌紫杉醇耐药细胞株A2780 Taxol细胞耐药性的逆转作用及机制。方法: 氯化两面针碱干预A2780 Taxol后

应用MTT比色法检测多柔比星(adriamycin

ADM)

依托泊苷(etoposide

VP-16)

羟基喜树碱(hydroxycamptothecin

HCPT)对A2780Taxol细胞的抑制率;实时荧光定量PCR检测不同给药组对多药耐药基因(MDR1 mRNA)表达的影响;HE染色法观察肿瘤细胞结构的改变;TUNEL法分析其对细胞凋亡的影响。结果: 耐药细胞A2780 Taxol 对ADM

VP-16

HCPT的耐药倍数分别为1.87

1.07

3.25;经氯化两面针碱干预后

降低了A2780 Taxol细胞对抗肿瘤药ADM

VP-16

HCPT的IC50值

可下降A2780 Taxol细胞的多药耐药(MDR1)基因的表达

HE染色可见NC给药组改变了A2780 Taxol细胞的形态结构

TUNEL染色凋亡细胞明显增多

凋亡指数为(58.03±1.46)%。结论: 氯化两面针碱能有效逆转A2780 Taxol多药耐药性

具有良好的抗肿瘤药物多药耐药逆转作用。

Abstract

Objective: To investigate the reversal or effect of multi-drug resistance by nitidine chloride(NC) on A2780 Taxol cell. Method: The reversal efficacy of NC was determined by MTT assay. The expressionof multidrug resistance 1 (MDR1) mRNA was assessed by reverse transcription-polymerase chain reaction (RT-PCR).The structural changes of A2780 Taxol cell were examined with hematoxylin and eosin staining(HE).The apoptosis of A2780 Taxol cell was detected by TUNEL assay. Result: The resistance fold of A2780 Taxol cell was 1.87

1.07

3.25 for adriamycin(ADM)

etopaside(VP-16)

hydroxycamptothecin(HCPT).The 50% inhibition concentration(IC50) value of A2780 Taxol cell was reduced by NC(0.375

0.75

1.5 mg·L-1) combined with ADM

VP-16 or HCPT

and NC could decrease the expression of MDR1-mRNA

change the structural of A2780 Taxol cell. The apoptosis index (AI) was(58.03±1.46)% detected by TUNEL assay. Conclusion: Nitidine chloride can reverse the multidrug resistance of A2780 Taxol cell.

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