WANG Jie-qiong, XU Li-li, ZHANG Li-na, et al. Effect of Shuyu Capsule on Expression of μ-opioid Receptors in Hypothalamus of Premenstrual Syndrome of Liver-Qi Stagnation Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 105-108.
DOI:
WANG Jie-qiong, XU Li-li, ZHANG Li-na, et al. Effect of Shuyu Capsule on Expression of μ-opioid Receptors in Hypothalamus of Premenstrual Syndrome of Liver-Qi Stagnation Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 105-108. DOI: 10.13422/j.cnki.syfjx.2015130105.
Effect of Shuyu Capsule on Expression of μ-opioid Receptors in Hypothalamus of Premenstrual Syndrome of Liver-Qi Stagnation Rats
Objective: To observe the effect of Shuyu capsule on μ-opioid receptors (MOR) expression in the hypothalamus of premenstrual syndrome (PMS) with liver-qi stagnation model rats
and to investigate its possible mechanism. Method: Rats were selected by using an open field test and vaginal smear in this experiment. The selected rats were divided into 4 groups:the normal group
the model group
the Shuyu capsule group(0.408 g·kg-1) and the naloxone group(0.4 mg·kg-1). The PMS with liver-qi stagnation model in rats was prepared by a chronic bondage stress method. The distribution and protein level of MOR in hypothalamus of rats were observed by immunofluorescence chemical technology and Western blot. Result: Compared with the normal group
the scores of open field test decreased
the distribution of messy-arranged MOR in hypothalamus increased
MOR protein expression increased in the model group (P<0.01). Compared with the model group
the scores of open field test increased
the distribution of messy-arranged MOR in hypothalamus decreased
MOR protein expression decreased in the Shuyu capsule and naloxone groups (P<0.01). Conclusion: Shuyu capsule could improve the symptoms of PMS with liver-qi depression syndrome
which may be achieved by regulating the MOR in the hypothalamus. In addition
the up-regulation of MOR in the central nervous system may be one of the pathophysiologic mechanisms.
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