Protective Effects and Mechanisms Research of Xinjiangtang Granules on Kidney in Type 2 Diabetic Rats

LYU Xiao-long; YANG Yuan-sheng; CHEN Ken; WANG Hui; ZHOU Wen; LIU Shao-bo

doi:10.13422/j.cnki.syfjx.2015130130

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Protective Effects and Mechanisms Research of Xinjiangtang Granules on Kidney in Type 2 Diabetic Rats

更新时间：2024-01-04

- Protective Effects and Mechanisms Research of Xinjiangtang Granules on Kidney in Type 2 Diabetic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 13, Pages: 130-134(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015130130
  CLC： R285.5
- Published：2015
- 稿件说明：
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LYU Xiao-long, YANG Yuan-sheng, CHEN Ken, et al. Protective Effects and Mechanisms Research of Xinjiangtang Granules on Kidney in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 130-134.
DOI：

LYU Xiao-long, YANG Yuan-sheng, CHEN Ken, et al. Protective Effects and Mechanisms Research of Xinjiangtang Granules on Kidney in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 130-134. DOI： 10.13422/j.cnki.syfjx.2015130130.

摘要

目的: 探讨新降糖颗粒对2型糖尿病(T2DM)大鼠肾脏保护作用及其作用机制。方法: 50只雄性SD大鼠分为正常组(8只

普通饲料)

其余大鼠采用高脂饲料喂养4周后

一次性腹腔注射链脲佐菌素(STZ)40 mg·kg-1建立2型糖尿病模型大鼠

随机分为模型组

二甲双胍组(0.15 g·kg-1)

新降糖颗粒高、低剂量组(12.64

6.32 g·kg-1)

每天ig给药1次。干预8周后

检测大鼠体重

血清中空腹血糖(FBG)

尿素氮(BUN)

血肌酐(SCr)

总胆固醇(TC)

甘油三酯(TG)

高密度脂蛋白-胆固醇(HDL-C)

低密度脂蛋白-胆固醇(LDL-C);肾脏组织中超氧化物歧化酶(SOD)

丙二醛(MDA)

谷胱甘肽过氧化物酶(GSH-Px)

过氧化氢酶(CAT)的表达量及RT-qPCR检测转化生长因子-β1(TGF-β1)

纤溶酶原激活物抑制物(PAI-1)的表达量。结果: 与正常组比较

模型组大鼠FBG

BUN

SCr

LDL-C

肾脏MDA水平及PAI-1

TGF-β1 mRNA表达量明显升高(P<0.01)

大鼠体重明显降低

HDL-C及肾脏SOD

GSH-Px

CAT水平明显降低(P<0.01);与模型组比较

新降糖颗粒能显著降低大鼠FBG

BUN

SCr

LDL-C

肾脏MDA水平及PAI-1

TGF-β1 mRNA表达量(P<0.05

P<0.01);增加大鼠体重

升高HDL-C及肾脏SOD

GSH-Px

CAT水平(P<0.05

P<0.01)。结论: 新降糖颗粒能有效改善糖尿病肾病(diabetic nephropathy

DN)大鼠的症状

推测其机制可能与新降糖颗粒抑制TGF-β1

PAI-l mRNA的表达、减轻肾脏氧化应激、改善肾功能等多因素相关。

Abstract

Objective: To investigate the protective effects and mechanisms of Xinjiangtang granule on kidney in streptozotocin-induced type 2 diabetic mellitus (T2DM) rats. Method: Fifty male SD rats were randomly divided into the normal control group (8 rats

normal diet) and the model group (42 rats). Rats in the model group received a high-fat-diet for 4 weeks and peritoneal injection of streptozotocin (STZ

40 mg·kg-1) to induce T2DM. The T2DM rats were randomly subdivided into the model group

the metformin group (0.15 g·kg-1)

the high-and low-dose Xinjiangtang granule groups (12.64

6.32 g·kg-1). The corresoponding medicines were intragastrically administered to rats once daily for 8 weeks. After 8-week treatment

body weight

fasting blood glucose (FBG)

ureanitrogen (BUN)

serum creatinine (SCr)

total cholesterol (TC)

triglyceride(TG)

high density lipoprotein cholesterol (HDL-C)

low density lipoprotein cholesterol (LDL-C) of serum

superoxide dismutase (SOD)

malondialdehyde (MDA)

glutathione peroxidase (GSH-Px)

catalase (CAT) and transforming growth factor-β1(TGF-β1) mRNA

plasminogen activator inhibitor-1(PAI-1) mRNA of kidney were detected. Result: Compared with the normal group

the levels of FBG

BUN

SCr

LDL-C

MDA and mRNA expressions of PAI-1

TGF-β1 increased

the body weight

levels of HDL-C

SOD

GSH-Px and CAT decreased in the model group (P<0.05

P<0.01). Compared with model group

the levels of FBG

BUN

SCr

LDL-C

MDA and mRNA expressions of PAI-1

TGF-β1 decreased

the body weight

levels of HDL-C

SOD

GSH-Px and CAT increased in the Xinjiangtang granule groups (P<0.05

P<0.01). Conclusion: Xinjiangtang granule could remarkably regulate glycometabolism of T2DM rats

and mechanism may be associated with inhibiting the mRNA expressions of TGF-β1 and PAI-l

weakening oxidative stress and improving kidney function.

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