Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity

ZHENG Yu-xiang; XIAO Feng-xia; LIN Li; CHEN Kang; WANG Zhen-hua; TIAN Jun; SONG Jian-ping; WANG Qi

doi:10.13422/j.cnki.syfjx.2015140008

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Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity

更新时间：2024-01-04

- Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 14, Pages: 8-11(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015140008
  CLC： R285;R284.2
- Published：2015
- 稿件说明：
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ZHENG Yu-xiang, XIAO Feng-xia, LIN Li, et al. Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 8-11.
DOI：

ZHENG Yu-xiang, XIAO Feng-xia, LIN Li, et al. Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 8-11. DOI： 10.13422/j.cnki.syfjx.2015140008.

摘要

目的: 优选青蒿渣中总多糖的提取工艺参数并探究其抗氧化能力。方法: 以液料比、提取温度、提取时间为自变量

总多糖得率为因变量

利用响应面法优选青蒿渣总多糖的超声提取工艺。通过清除1

1-二苯基-2-苦基肼自由基、清除羟自由基及油脂抗氧化试验

与抗坏血酸的抗氧化能力进行比较

评价青蒿渣总多糖的抗氧化能力。结果: 最佳提取工艺条件为液料比30 :1

提取时间30 min

提取温度70 ℃;青蒿渣总多糖提取率1.773 3%。青蒿渣总多糖的抗氧化能力随质量浓度的增大而增强

其清除羟自由基的能力较抗坏血酸强

两者的半抑制浓度分别为(164.26±0.84)

(214.89±0.18) mg ·L-1。结论: 优选的提取工艺稳定可靠

青蒿渣总多糖具有一定抗氧化能力

为青蒿资源的综合利用提供参考。

Abstract

Objective: To optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue and investigate its antioxidant activity. Method: Response surface methodology was employed to optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue by taking liquid-solid ratio

extracting temperature and time as factors.Antioxidant activity of total polysaccharides was evaluated through several in vitro antioxidant assays

such as 1

1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity

hydroxyl radical scavenging activity and anti-lipid peroxidation with vitamin C as a reference. Result: Optimum extraction conditions of total polysaccharides were as follows:liquid-solid ratio of 30: 1

extraction time of 30 min and extraction temperature of 70 ℃.Under these conditions

yield of total polysaccharides could be up to 1.773 3%.Antioxidant capacity of total polysaccharides increased with the concentration of total polysaccharides increased

its scavenging ability towards hydroxyl free radical was stronger than vitamin C. Conclusion: Optimized extraction process is stable and reliable

total polysaccharides from Artemisiae Annuae Herba residue has antioxidant capacity

which can provide a reference for comprehensive utilization of Artemisiae Annuae Herba.

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