Separation and Purification of Polysaccharides from Phellodendri Amurensis Cortex and Effect on Proliferation Function of Mice Spleen Lymphocyte

XUE Juan; ZENG Wei-min; WANG Chang-fu; ZHANG Hong-wei; WANG Qiu-hong

doi:10.13422/j.cnki.syfjx.2015140044

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Separation and Purification of Polysaccharides from Phellodendri Amurensis Cortex and Effect on Proliferation Function of Mice Spleen Lymphocyte

更新时间：2024-01-04

- Separation and Purification of Polysaccharides from Phellodendri Amurensis Cortex and Effect on Proliferation Function of Mice Spleen Lymphocyte
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 14, Pages: 44-48(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015140044
  CLC： R284.1;R285.5
- Published：2015
- 稿件说明：
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XUE Juan, ZENG Wei-min, WANG Chang-fu, et al. Separation and Purification of Polysaccharides from Phellodendri Amurensis Cortex and Effect on Proliferation Function of Mice Spleen Lymphocyte[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 44-48.
DOI：

XUE Juan, ZENG Wei-min, WANG Chang-fu, et al. Separation and Purification of Polysaccharides from Phellodendri Amurensis Cortex and Effect on Proliferation Function of Mice Spleen Lymphocyte[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 44-48. DOI： 10.13422/j.cnki.syfjx.2015140044.

摘要

目的: 分离纯化关黄柏总多糖

研究纯化组分对小鼠脾淋巴细胞增殖的影响。方法: 采用DEAE Cellulose 52

DEAE-Sepharose F.F

Sephacryl S-400等载体对关黄柏总多糖进行分离纯化

高效凝胶过滤色谱法鉴定纯度并测定分子量

PMP 柱前衍生化测定单糖组成;采用MTT法测定各多糖组分对ConA诱导的小鼠脾淋巴细胞增殖的作用。结果: 关黄柏总多糖占药材的4.3%

从关黄柏总多糖中分离纯化得到3种多糖组分G1

分别占总多糖的6%

10%

经HPLC鉴定证明为均一多糖

均主要含阿拉伯糖、鼠李糖、半乳糖和半乳糖醛酸

其摩尔比分别是G1(1 :1.06 :1.04 :1.14)

G2(1 :1.27 :1.10 :1.09)

G3(1 :1.23 :0.99 :1.55)

3种均一多糖对ConA诱导的T淋巴细胞增殖有显著的抑制作用。结论: 3种均一关黄柏总多糖主要由阿拉伯糖、鼠李糖、半乳糖和半乳糖醛酸组成

具有一定显著的抑制细胞免疫的作用

为进一步研究提供了实验基础。

Abstract

Objective: To separate and purificate homogeneous polysaccharides from Phellodendri Amurensis Cortex and study the effect on proliferation function of mice spleen lymphocyte transformation. Method: The DEAE Cellulose 52

DEAE-Sepharose F.F and Sephacryl S-400 columns were used to separate and purificate homogeneous polysaccharides and the monosaccharide composition and structure were preliminarily identificated by periodate oxidation

smith degradation

methylation

HPLC and GC-MS

and their effects on proliferation function of mice spleen lymphocyte transformation were determinated by MTT. Result: The yield of crude polysaccharide was 4%. Three homogeneous polysaccharides were separated and purificated (G1-G3)

the yields of purified polysaccharides were 6%

and 10%

espectively

which were identified as homogeneous polysaccharides by HPLC. They mainly contained arabinose

rhamnose

galactose and galacturonic acid

the mole ratios were 1: 1.06: 1.04: 1.14 (G1)

1: 1.27: 1.10: 1.09 (G2) and 1: 1.23: 0.99: 1.55 (G3)

and the homogeneous polysaccharides were able to inhibit the proliferation of lymphocytes induced by ConA. Conclusion: The homogeneous polysaccharides contained arabinose

rhamnose

galactose and galacturonic acid

and showed inhibit effect of cellular immunity

which established the experimental basis for the further study of the polysaccharides.

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