Objective: To establish an HPLC method for simultaneous determination ofrutin

hyperin

isoquercitrinand quercetinin Mone Flose dispensing granule. Method: HPLC method was performed on a Phenomenex C18(4.6 mm×250 mm

5 μm)with Acetonitrile(A)-0.1% phosphoric acid(B) as mobile phase by gradient elution. The flow rate was 1.0 mL · min

column temperature was 30 ℃and detection wavelength was 360 nm. Result: Rutin

hyperin

isoquercitrinand quercetin showed good linearity in the ranges of 0.230 2-2.877 5 μg(r=0.999 2)

0.134 8-1.685 0 μg(r=0.999 5)

0.195 4-2.442 5 μg(r=0.999 1) and 0.033 8-0.422 5 μg(r=0.999 9) with average recoveries of 98.39%

98.71%

100.00% and 100.57%

with RSD 1.7%

1.4%

1.8% and 2.0%

respectively. Conclusion: The method is rapid

accurate and repeatable

which can provide a reliable way for evaluating the quality of Mone Flose dispensing granule.