Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats

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Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats

更新时间：2024-01-04

- Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 14, Pages: 92-96(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015140092
  CLC： R285.5
- Published：2015
- 稿件说明：
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WU Liang-bing, PAN Yi, YANG Tao, et al. Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 92-96.
DOI：

WU Liang-bing, PAN Yi, YANG Tao, et al. Effect of Tiaoganshen Qutanyu-formula on Signal Pathway of TGF--Smads of Heart and Kidney in Spontaneous Hypertensive Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 92-96. DOI： 10.13422/j.cnki.syfjx.2015140092.

摘要

目的: 探讨“调肝肾

祛痰瘀”复方血压峰值前给药对自发性高血压大鼠(SHR)心肾组织转化生长因子-β1 (TGF-β1)-Smads信号通路的影响。方法: 将30只12周龄的自发性高血压大鼠

随机分为复方组(4 g ·kg-1)

氯沙坦组 (30 mg ·kg-1)和模型组

另设SD大鼠10只为正常组

12周后

采用RT-qPCR检测心肾组织Smad2

Smad3和Smad7 mRNA表达

免疫组化法检测肾组织Smad2

Smad3和Smad7蛋白表达。结果: 与正常组比较

模型组肾脏Smad2

Smad3和心脏TGF-β1

Smad2

Smad3的mRNA表达明显升高

心脏和肾脏Smad7的mRNA表达明显降低(P<0.01)

肾组织Smad2和Smad3的蛋白表达均明显升高

Smad7蛋白表达明显降低(P<0.01);与模型组比较

复方组能够下调肾脏Smad2

Smad3和心脏TGF-β1

Smad3的 mRNA表达

上调肾脏和心脏Smad7的mRNA表达;复方组转化生长因子β1(TGF-β1)mRNA表达的下调程度比氯沙坦组明显 (P<0.05);同时复方组的Smad2和Smad3的蛋白表达均明显下调(P<0.01)

Smad7蛋白表达均明显上调(P<0.01)

其中

复方组的Smad7蛋白表达与正常组无统计学差异。结论: 调肝肾祛痰复方血压峰值前给药能够明显减少ECM聚积而减少高血压心肾纤维化。其作用机制应是在中医阴阳升降机制协同下

通过下调心肾的Smad2

Smad3和上调Smad7的表达以阻断TGF-β1-Smads信号转导通路所致。

Abstract

Objective: To observe the effect of Tiaoganshen Qutanyu-formula (TQF) on the pathway of transforming growth factor-β1(TGF-β1)-Smad2/3/7 in the kidney of spontaneous hypertensive rats (SHR) administered before blood pressure peak. Method: A total of 30 12-week male SHR were randomly divided into 3 groups:the TQF group (4 g · kg-1)

the losartan group (30 mg · kg-1) and the normal group. Another healthy 10 SD rats were taken as the normal group. The mRNA expressions of Smad2

Smad3 and Smad7 in the heart and kidney were detected by RT-PCR

and the protein expressions of Smad2

Smad3 and Smad7 in the kidney were determined by munohistochemical method after 12 weeks of treatment. Result: Compared with the normal group

the mRNA expressions of Smad2

Smad3 in the kidney and TGF-β1

Smad2

Smad3 in the heart increased

the mRNA expression of Smad7 in both kidney and heart decreased

the protein expressions of Smad2

Smad3 in the kidney increased

the protein expression of Smad7 decreased in the model group (P<0.01). Compared with model group

the mRNA expressions of Smad2

Smad3 in the kidney and TGF-β1

Smad2

Smad3 in the heart decreased

the mRNA expression of Smad7 in both kidney and heart increased

the protein expressions of Smad2

Smad3 in the kidney decreased in the TQF group (P<0.05

P<0.01). There was no statistical difference for protein expression of Smad7 between the TQF group and the normal group. Conclusion: TQF taken before blood pressure peak could reduce the hypertensive heart and kidney fibrosis by decreasing the aggregation of extra cellular matrix. The mechanism might be the synergistic effect of Yin-yang theory of the TGF-β1-Smads signal pathway interruption by regulating the Smad2

Smad3 and Smad7 in the heart and kidney tissue.

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