Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice

XU Yue; GE Hui-qi; GAO Hui; ZHAO Min-jie; JIA Tian-zhu

doi:10.13422/j.cnki.syfjx.2015140116

您当前的位置：

首页 >

文章列表页 >

Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice

更新时间：2024-01-04

- Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 14, Pages: 116-119(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015140116
  CLC： R285.5
- Published：2015
- 稿件说明：
移动端阅览
XU Yue, GE Hui-qi, GAO Hui, et al. Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 116-119.
DOI：

XU Yue, GE Hui-qi, GAO Hui, et al. Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(14): 116-119. DOI： 10.13422/j.cnki.syfjx.2015140116.

摘要

目的: 比较五味子不同炮制品对小鼠脾淋巴细胞增殖作用的影响

为五味子临床合理用药提供实验依据。方法: 将五味子不同质量浓度 (400

200

100

50 mg ·L-1) 提取液与细胞密度为4×106个/mL的脾淋巴细胞于96孔板中共同培养48 h

每组6个复孔

以增殖率为指标

采用CCK-8比较生五味子、酒五味子、醋五味子对小鼠脾淋巴细胞、刀豆蛋白A(Con A)和脂多糖(LPS)诱导的T

B淋巴细胞增殖的影响。结果: 与空白组比较

Con A

LPS可诱导T

B淋巴细胞增殖;与不同指标相应空白组比较

五味子给药组均能不同程度促进未活化脾淋巴细胞及ConA和LPS诱导的T

B淋巴细胞的增殖

同等剂量下

酒五味子增殖效果最佳

优于生五味子、醋五味子(P<0.05

P<0.01)。结论: 五味子酒制后对小鼠淋巴细胞增殖作用显著增强

符合“入补药熟用”传统理论。

Abstract

Objective: To compare Schisandrae Chinensis Fructus prepared with different processing methods on splenic lymphocyte proliferation in mice of. Method: The splenic lymphocyte cells were cultured with different concentrations(400

200

100

50 mg · L-1) of Schisandrae Chinensis Fructu extraction in 96-well plate

each concentration was repeated for six times. After 48 hours

the cell proliferation rate of spleen cells and T/B lymphocytes were measured by the CCK-8 colorimetric method.The T lymphocyte was induced by concanavalin A (Con A)and B lymphocyte was induced by lipopolysaccharide (LPS). Result: Compared with the blank group

Con A

LPS could induce T lymphocyte and B lymphocyte. Compared with blank group in terms of other corresponding indicators

different concentrations of Schisandrae Chinensis Fructus extraction could increase proliferation rate

especially in wine steaming Schisandrae Chinensis Fructus group with the same dosage

wine steaming Schisandrae Chinensis Fructus was better than crude Schisandrae Chinensis Fructus and vinegar steaming Schisandrae Chinensis Fructs. Conclusion: Wine steaming Schisandrae Chinensis Fructus could obviously increase the cell proliferation rate

the result is accordance with TCM theory of processed Schisandra Chinensis Fruits as Tonic.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Herbal Textual Research on Zanthoxylum armatum and Zanthoxyli Radix in Famous Classical Formulas

Color Space Method Combined with Chemometrics to Determine Processing Degree of Angelicae Sinensis Radix Carbonisata

Maillard Reaction in Processing of Traditional Chinese Medicine： A Review

Herbal Textual Research on Houttuyniae Herba in Famous Classical Formulas

Analyzing Differences in Volatile Components of Citri Reticulatae Pericarpium Before and After Being Stir-fried with Halloysitum Rubrum Based on HS-GC-MS and Intelligent Sensory Technology

Related Author

ZENG Zhen

LIU Yanmeng

WANG Yihan

WANG Yapeng

HAO Erwei

YAO Chun

ZHAN Zhilai

QIN Liuying

Related Institution

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica， Guangxi University of Chinese Medicine

Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granules in Enterprises，Guangdong Efong Pharmaceutical Co. Ltd.

Shandong University of Traditional Chinese Medicine

Shandong Academy of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰