Objective: To study effect of oleanolic acid on apoptosis related proteins expression in H22 mice tumor. Method: Mice H22 tumor-burdened tumor model was used in vivo 50 mice inoculated H22 cell lines were randomly divided into model group

cyclophosphamide group

oleanolic acid low

medium and high dose groups(1.25

2.50

5.00 mg · kg-1). The detection of liver

body weight ratio and spleen index was performed. Immunohistochemical method was used to detect the expression of survivin and B cell lymphoma/leukemia-2 (Bcl-2) protein

in situ end labeling method was used to detect apoptosis index

and analyzes its relevance. Result: Oleanolic acid could improve H22 tumor-burdened mice liver and body ratio and spleen index

the survivin protein expression was significantly lower than that in the model group(P<0.01);oleanolic acid high dose group decreased significantly Bcl-2 protein expression compared with model group(P<0.01);apoptosis index was significantly increased compared with model group (P<0.01). Conclusion: Oleanolic acid can inhibit H22 mice tumor-burdened tumor cells

increase the rate of apoptosis

thus promotes apoptosis. Survivin and Bcl-2 abnormal expression is closely related to the occurrence of tumor

play a role of anti-tumor by regulating apoptosis together.