Objective: To study the effects of Bushen Shugan formula (BSSGF) on human lung cancer A549 cells and related stem genes. Method: SD rats were randomly divided into 6 groups:the normal group (normal saline)

the cisplatin group (8 g · kg-1)

the low-and high-dose BSSGF groups (15

30 g · kg-1)

the low-dose BSSGF plus cisplatin group and the high-dose BSSGF plus cisplatin group of 10 rats each. The rats received the corresponding medicines and the serum containing drugs were prepared. A549 cells were cultured in the drug serum with different concentrations. The cell proliferation was detected by CCK8 assay.The cell cycle was tested by the flow cytometry. The cell apoptosis was detected by using Annexin V/PI staining with flow cytometry. The related gene expression was detected by using quantitative PCR. The protein expression of CD133 was tested by using flow cytometry. Result: BSSGF could inhibit the proliferation of A549 in a time-and dose-dependent manner and had synergies when combined with cisplatin. The inhibition rate was 45.39%

54.76% and 59.94%

respectively

after 24

48 and 72 h incubation BSSGF could induce the cell apoptosis

the cell percentage in G2/M increased with the increasing of concentration. BSSGF combined with cisplatin could reduce the gene expressions of CD133

SOX-2

OCT-4

and lowered the protein expression of CD133. Conclusion: BSSGF could inhibit the cell proliferation and promote the cell apoptosis of A549 cells. Combined with cisplatin

BSSGF could reduce the expression of stem factors

such as CD133

SOX-2 and OCT-4.