Objective: Taking drug dissolution and absorption simulating system (DDASS) to evaluate in vitro release and trans-membrane characteristics of Leigongteng bilayer tablets

and study in vivo and in vitro correlation between DDASS and the preparation in Beagle dogs. Method: With wilfordine as the index component

release characteristics of Leigongteng tablets and Leigongteng bilayer tablets were evaluated by the rotating basket method

in order to study in vitro continuous

dynamic

real-time release and trans-membrane characteristics in DDASS model as well as pharmacokinetic characteristics of these two preparations in Beagle dogs and their corresponding in vivo and in vitro correlation. Result: Release processes of wilfordine in Leigongteng bilayer tablets in the rotating basket method and DDASS conformed to the first-order kinetic equation and the skeleton corrosion mechanism.Compared with Leigongteng tablets

Leigongteng bilayer tablets showed obvious sustained release of wilfordine in Beagle dogs with the relative bioavailability of 174.88%.In DDASS

release/trans-membrane characteristics was significantly correlated to dog absorption characteristics

which was superior to the correlation between dissolution of the rotating basket method and Beagle dog absorption characteristics. Conclusion: DDASS model can effectively evaluate in vitro release kinetics and in vivo absorption kinetics of wilfordine in Leigongteng bilayer tablets.