Optimization of Stir-frying Processing Technology of Bupleuri Radix with Turtle Blood by Orthogonal Test

YU Huan; LI Xiao-ning; ZHONG Ling-yun; ZHANG Jin-lian; NING Xi-xian; YANG Qiang; GONG Qian-feng

doi:10.13422/j.cnki.syfjx.2015150008

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Optimization of Stir-frying Processing Technology of Bupleuri Radix with Turtle Blood by Orthogonal Test

更新时间：2024-01-04

- Optimization of Stir-frying Processing Technology of Bupleuri Radix with Turtle Blood by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 15, Pages: 8-11(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015150008
  CLC： R283
- Published：2015
- 稿件说明：
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YU Huan, LI Xiao-ning, ZHONG Ling-yun, et al. Optimization of Stir-frying Processing Technology of Bupleuri Radix with Turtle Blood by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 8-11.
DOI：

YU Huan, LI Xiao-ning, ZHONG Ling-yun, et al. Optimization of Stir-frying Processing Technology of Bupleuri Radix with Turtle Blood by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 8-11. DOI： 10.13422/j.cnki.syfjx.2015150008.

摘要

目的: 优选鳖血柴胡的炮制工艺。方法: 以柴胡皂苷a

d及醇溶性浸出物含量的综合评分为指标

通过正交试验考察炮制时间、鳖血用量、炮制温度对鳖血柴胡炮制工艺的影响。采用HPLC测定柴胡皂苷a

d的含量

流动相乙腈(A)-水(B)梯度洗脱(0~50 min

25%~90%A;50~55 min

90%A)

检测波长210 nm。结果: 炒制时间对炮制工艺具有显著性影响。最佳炮制工艺为150 ℃炮制10 min

加鳖血量0.05 mL ·g-1。醇溶性浸出物及柴胡皂苷a

d质量分数分别为12.42%

0.85%

0.23%

0.90%。结论: 优选的炮制工艺合理可靠、重复性好

为规范鳖血柴胡饮片的炮制加工提供参考。

Abstract

Objective: To optimize processing technology of Bupleuri Radix with turtle blood. Method: With composite score of saikosaponin a

d and ethanol soluble extractives as index

an orthogonal test was adopted to investigate influence of processing time

turtle blood amount and processing temperature on processing technology of Bupleuri Radix processed with turtle blood.HPLC was adopted to determine contents of saikosaponin a

d and eluted in gradient with mobile phase of acetonitrile-water

detection wavelength was at 210 nm. Result: Stir-frying time had a significant influence on processing technology.According to optimum processing technology

processing temperature was set at 150 ℃

processing time was 10 min

the amount of turtle blood was 0.05 mL · g-1.Mass fractions of ethanol soluble extractives and saikosaponin a

d were 12.42%

0.85%

0.23%

0.90%

respectively. Conclusion: This optimized processing technology is reasonable

reliable and highly reproducible

it can provide a reference for processing of Bupleuri Radix processed with turtle blood.

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