Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC

LI Qing-song; DENG Xiao-xia; ZENG Wen-xue; LI Shan-shan; PENG Le; LIU Hong-ning; LIN Se-qi

doi:10.13422/j.cnki.syfjx.2015150044

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Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC

更新时间：2024-01-04

- Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 15, Pages: 44-46(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015150044
  CLC： R285.5
- Published：2015
- 稿件说明：
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LI Qing-song, DENG Xiao-xia, ZENG Wen-xue, et al. Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 44-46.
DOI：

LI Qing-song, DENG Xiao-xia, ZENG Wen-xue, et al. Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 44-46. DOI： 10.13422/j.cnki.syfjx.2015150044.

摘要

目的: 建立微透析联合HPLC测定荷瘤小鼠皮下微透析液中乳酸含量的方法。方法: 采用微透析系统收集正常小鼠和H22荷瘤小鼠皮下微透析液

利用HPLC测定小鼠皮下微透析液中乳酸的含量。ZORBAX SB-Aq色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%磷酸溶液(5 :95)

流速1.0 mL ·min-1

检测波长210 nm柱温30 ℃。结果: 线性范围20.39~611.6 mg ·L-1。平均回收率97.41%

RSD 4.6%。结论: 该方法能够准确检测出透析液中乳酸的含量

具有简单可靠、分析速度快、灵敏度高、精密度好等优点

为乳酸的检测提供新途径。

Abstract

Objective: To establish a method for the determination of lactic acid content in subcutaneous micro-dialysis fluid of hepatoma-bearing mice by micro-dialysis combined with HPLC. Method: Micro-dialysis system was adopted to collect subcutaneous micro-dialysis fluid of normal mice and tumor-burdened mice for determining the content of lactic acid in subcutaneous micro-dialysis fluid by HPLC.The ZORBAX SB-Aq column (4.6 mm×150 mm

5 μm) was used with the mobile phase of methanol-0.1% phosphoric acid solution (5: 95) at a flow rate of 1.0 mL · min-1

the detection wavelength was 210 nm

and the column temperature was set at 30 ℃. Result: The linearity ranged between 20.39-611.6 mg · L-1 with the average recovery rate of 97.41% and RSD of 4.1%. Conclusion: This method is simple

reliable

rapid and highly sensitive

it can accurately detect lactic acid content in dialyzate and provide a new way to detect lactic acid.

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Related Author

Hong-xing MA

Long-fei LIN

Yu-ling LIU

Sai FU

Jin-xin SHAO

Ji-zhong ZHU

Chuan-gui LIU

Hui LI

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

Jilin Huakang Pharmaceutical Co. Ltd.

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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