XIAO Hong-he, YAN Yan, YUE Xin-yi, et al. HPLC Fingerprints of Gegen Tang and Multi-target Component Quantitative Analysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 47-52.
DOI:
XIAO Hong-he, YAN Yan, YUE Xin-yi, et al. HPLC Fingerprints of Gegen Tang and Multi-target Component Quantitative Analysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 47-52. DOI: 10.13422/j.cnki.syfjx.2015150047.
HPLC Fingerprints of Gegen Tang and Multi-target Component Quantitative Analysis
Objective: To develop an HPLC-DAD fingerprint to conduct a quantitative analysis on the 14 index ingredients of Gegen Tang (GGD) and provide a method for the quality control for the water extracts from GGD. Method: The AgelaVenusil MP-C18was adopted and eluted in gradient with acetonitrile and water (consisting 0.1% formic acid) at 30 ℃. The flow rate was 1.0 mL · min-1
and the detection wavelength was set at 207
230
254 and 275 nm. Result: Totally 38 common peaks were marked and 10 were identified in this fingerprint
with a good similarity (≥0.978) and less difference among the 11 batches. Specifically
14 index components in the 11 batches of GGD feature even and stable content
stable sample preparation method and controllable quality. Conclusion: The combination of fingerprint and quantitative analysis can roundly reflected the quality of GGD and can be taken as an effective method for controlling the quality of the water extracts from GGD.
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Related Author
LI Wen-jing
HONG Bo
ZHANG Zhen-shan
YAN Ping
TAN Zhi-bin
LIN Wen-hua
TONG Jia-yun
ZHAN Ruo-ting
Related Institution
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Modern Research Center for Traditional Chinese Medicine,Shanxi University
Hubei Meisichuang Pharmaceutical Co. Ltd.
School of Pharmacy, Henan University of Chinese Medicine
Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine, Henan University of Chinese Medicine