Simultaneous Determination of Seven Compounds in Fangfeng Tongsheng Pill by HPLC

GUO Qi; LI Xiang-yang; LI Gui-ben

doi:10.13422/j.cnki.syfjx.2015150079

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Simultaneous Determination of Seven Compounds in Fangfeng Tongsheng Pill by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Seven Compounds in Fangfeng Tongsheng Pill by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 15, Pages: 79-83(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015150079
  CLC： O657.72;R286.0
- Published：2015
- 稿件说明：
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GUO Qi, LI Xiang-yang, LI Gui-ben. Simultaneous Determination of Seven Compounds in Fangfeng Tongsheng Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 79-83.
DOI：

GUO Qi, LI Xiang-yang, LI Gui-ben. Simultaneous Determination of Seven Compounds in Fangfeng Tongsheng Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 79-83. DOI： 10.13422/j.cnki.syfjx.2015150079.

摘要

目的: 建立防风通圣丸中栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分的高效液相色谱测定方法。方法: 采用phenomenex Luna 5u-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇 (A)-0.1%磷酸溶液(B)

梯度洗脱

流速0.8 mL ·min-1

检测波长分别为234

254 nm。结果: 栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分在相应的范围内线性关系良好

r不低于0.999 1;平均回收率97.3%~99.8%;RSD均<2.0。结论: 经方法学验证

本方法简单、快速、灵敏、准确

可用于防风通圣丸中栀子苷、连翘苷、5-O-甲基维斯阿米醇苷、黄芩苷、黄芩素、汉黄芩素、甘草酸铵7个成分的含量测定。

Abstract

Objective: To develop an HPLC method for simultaneous determination of geniposide

phillyrin

5-O-methylvisammioside

baicalin

baicalein

wogonin

and ammonium glycyrrhizinate in Fangfeng Tongsheng pill. Method: The chromatographic separation was on phenomenex Luna 5u-C18(4.6 mm×250 mm

5 μm) with a mobile phase consisting of methanol (A)-0.1% phosphoric acid solution (B) in a gradient mode at a flow rate of 0.8 mL · min-1. The detection wavelengths were 234 nm and 254 nm. Result: Within the linear range of geniposide

phillyrin

5-O-methylvisammioside

baicalin

baicalein

wogonin

ammonium glycyrrhizinate

the correlation coefficients were lager than 0.999 1.The average recoveries of the seven components were in the range of 97.3%-99.8%. RSD were less than 2.0%. Conclusion: By method validation

this method is proved to be simple

rapid

sensitive and accurate. It can be used to determine the contents of geniposide

phillyrin

5-O-methylvisammioside

baicalin

baicalein

wogonin

and ammonium glycyrrhizinate in Fangfeng Tongsheng pill.

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