Objective: To study pharmacokinetic characteristics on paeoniflorin in Xuefu Zhuyu Tang in rats. Method: The paeoniflorin content in serum of rats was determined by HPLC.Diamonsil C18 column (4.6 mm×250 mm

5 μm) was adopted and eluted with the mobile phase of acetonitrile-water (16: 84).The flow rate was 1.0 mL · min-1

the temperature of column was 30 ℃

the detection wavelength was 230 nm

and the sample size was 20 μL with riboflavin as the internal standard. Result: The linear relationship was good with the paeoniflorin concentration at 0.01-1.0 mg · L-1.The limit of quantitation was 0.01 mg · L-1.The average intra-day precision was 3.3%

while the average inter-days precision was 3.9%

the average recovery rate was 101.3%.After rats were administered with Xuefu Zhuyu Tang

the peak concentration of paeoniflorin was(0.363±0.080) mg · L-1

with Tmax of (0.276±0.084) h

Ka of (30.905±10.114) h-1

Ke of (1.638±0.181) h-1

t1/2 of (0.501±0.038) h

CL/F of (69.846±4.624) L · h-1 · kg-1

Vz/F of (36.521±12.287) L · kg-1and AUC0-t of (0.356±0.024) mg · L-1 · h. Conclusion: Paeoniflorin in Xuefu Zhuyu Tang is rapidly absorbed

distributed in a large volume and short in half-life

this study can provide a reference for studying effect of compatibility on paeoniflorin's pharmacokinetics and efficacy of Xuefu Zhuyu Tang.