Objective: To observe the anti-tumor effect and pathological morphology change of phenolic alkaloids of menispermum dauricum (PAMD) in pancreatic cancer cell line BxPC-3 transplanted into nude mice. Method: The 40 metastatic tumor model of tumor-bearing mice was established by injecting BxPC-3 cell line hypodermically at right oxter. After 24 h

the mice were randomly divided into 5 groups:the normal group (normal saline)

the model group (normal saline)

the 5-FU group (20 mg · kg-1)

the high-and low-dose PAMD groups (20

10 mg · kg-1) of 8 mice each. The mice except the normal group received heterotopic tumor transplantation of pancreatic carcinoma cell BxPC-3 subcutaneously. The corresponding medicines were intraperitoneally administered to mice for 20 days. Histomorphology changes of transplanted tumor tissue were observed by using HE staining. The ultrastructure of areas was observed by using electronicmicroscopy. Result: Compared with the normal group the tumor growth of tumor-bearing mice increased

pathological morphology and ultrastructure had obvious changes in the model group (P<0.01). Compared with the model group

the tumor growth inhibited

the inhibition rates were 51.67%

37.26%

33.58%

respectively

in the high-

low-dose PAMD and 5-FU groups (P<0.05). Moreover

pathological morphology and ultrastructure had good improvement. Conclusion: PAMD could effectively suppress tumor-bearing nude mice tumor cell growth of pancreatic cancer cell line BxPC-3 and improve the morphology of transplanted tumor tissue.