Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix

ZHOU Xun-rong; XU Ran; XIAO Wan; WANG Jian-ta; XIAO Hai-tao; TANG Lei

doi:10.13422/j.cnki.syfjx.2015150116

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Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix

更新时间：2024-01-04

- Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 15, Pages: 116-119(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015150116
  CLC： R284
- Published：2015
- 稿件说明：
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ZHOU Xun-rong, XU Ran, XIAO Wan, et al. Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 116-119.
DOI：

ZHOU Xun-rong, XU Ran, XIAO Wan, et al. Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(15): 116-119. DOI： 10.13422/j.cnki.syfjx.2015150116.

摘要

目的: 研究天葵子各提取部位体外抗氧化活性以及主要成分。方法: 将天葵子醇提物依次用乙酸乙酯和正丁醇萃取

分别得到乙酸乙酯层(TK-Y) 正丁醇层(TK-Z) 和水层(TK-S)

其中水层通过醇沉法得到水层多糖(TK-D)

并以维生素C(VC) 为对照

通过Fenton法和邻苯三酚法测定天葵子各部位和水层中的多糖对羟基自由基(·OH) 和超氧自由基(O2- ·) 的清除能力

研究天葵子的抗氧化活性

并以此为导向分离主要活性成分。结果: 天葵子抗氧化活性顺序为TK-D> TK-S> TK-Z> TK-Y

其中TK-D对超氧自由基的清除率与浓度呈正相关(Y=54.94X+27.15

R2=0.998 3)

IC50为0.42 g ·L-1;从正丁醇中分离得到5个化合物

分别鉴定为紫草氰苷(1)

东方唐松草苷(2)

格列风内酯(3)

β-谷甾醇(4)

胡萝卜苷(5)。结论: 首次证实了天葵子提取物中水层和正丁醇层是其抗氧化活性部位

其中水层中的抗氧化活性物质为多糖

化合物紫草氰苷

东方唐松草苷

格列风内酯是天葵子的主要化学成分。

Abstract

Objective: To study the antioxidant activity of different fractions and chemical constituents from Semiaquilegiae Radix in vitro. Method: Different extraction fractions from Semiaquilegiae Radix were ethyl acetate extraction(TK-Y)

n-butanol extraction(TK-Z)

water extraction(TK-S) and polysaccharide(TK-D). Under the chemical simulation conditions in vitro

the antioxidant activities of different extraction fractions and polysaccharide were determined by · OH

O2- · scavenging experiments. Furthermore

the chemical compounds were isolated from bio-active fraction. Result: The antioxidant activities of different extraction fractions from strong to weak were as follows:TK-D>TK-S>TK-Z>TK-Y.Scavenging activity of TK-D for O2- · and · OH were positively correlated (Y=54.94X+27.15

R2=0.998 3)

IC50=0.42 g · L-1.Five major components were isolated and identified from TK-Z as lithtospermoside(1)

thalictricoside(2)

griffonilide (3)

β-sitosterol(4)

daucosterol(5). Conclusion: The polysaccharide

n-butanol extraction and water extraction are active fractions and it identified the active fractions of Semiaquilegiae Radix for the first time. Lithtospermoside

thalictricoside and griffonilide were found as main contribution.

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