Mulberry Morus alba includes multiple polyhydroxylated alkaloidsi in leaves

branches root barks

which have many pharmacologic effects in reducing blood sugar and blood lipid

resisting virus

enhancing immunity and anti-aging. However

because spolyhydroxylated alkaloids share a similar structure with common carbohydrates in plants

with a strong polarity and alkalinity

they are hard to be separated and purified

which needs complicated separation procedures and multiple column chromatography. Furthermore

there is no literature about stationary phase materials for separating polyhydroxylated alkaloids. Therefore

the authors summarized the separation procedures for mulberry polyhydroxylated alkaloids

including reversed-phase liquid chromatography (RPLC)

normal phase liquid chromatography(NPLC)

ion exchange chromatography (IEC) and hydrophilic interaction chromatography (HILIC)

analyzed their advantages and disadvantages

and proposed the column chromatography method with carbosilane ionic liquid with a new-type adsorbing material. This new-type material is similar with zwitter-ion stationary phase in hydrophilic interaction chromatography but different in the ionic bond connecting zwitterions in the stationary phase

while showing the characteristic zwitter-ion separation

in order to provide reference for separating polyhydroxylated alkaloids from mulberry herbs and developing new-type mulberry drugs

successfully separate polyhydroxylated alkaloid and develop targeted drugs for patients with different types of diabetes. Finally

the authors defined the development and application prospect of polyhydroxylated alkaloids

which can modify the stationary phase in the chromatographic analysis by regulating the ionic liquid.