MO Mao-yan, ZHU Qiong-hua, XUE Xing-yang, et al. Urine Metabonomics Analysis of Dried and Charred Zingiberis Rhizoma Recens on Rats with Deficiency-cold Hemorrhagic Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 1-4.
DOI:
MO Mao-yan, ZHU Qiong-hua, XUE Xing-yang, et al. Urine Metabonomics Analysis of Dried and Charred Zingiberis Rhizoma Recens on Rats with Deficiency-cold Hemorrhagic Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 1-4. DOI: 10.13422/j.cnki.syfjx.2015160001.
Urine Metabonomics Analysis of Dried and Charred Zingiberis Rhizoma Recens on Rats with Deficiency-cold Hemorrhagic Disease
Objective: To investigate urine metabolic profile of rats with deficiency-cold hemorrhagic disease
and discuss intervention effects of dried and charred Zingiberis Rhizoma Recens. Method: Proton nuclear magnetic resonance (1H-NMR) technique was used to analyze metabolome in urine.Partial least squares discriminant analysis (PLS-DA) was processed to analyze metabonome difference among four groups.Seven potential biomarkers were selected by variable importance in the projection (VIP).Principal component analysis (PCA) was employed to process data from the charred Zingiberis Rhizoma Recens treatment group
hemostatic effects of charred Zingiberis Rhizoma Recens were investigated through the content of potential biomarkers. Result: Charred Zingiberis Rhizoma Recens could make disorder endogenous biomarkers of the model rats to normal levels
while dried Zingiberis Rhizoma Recens had weaker efficacy.Acetoacetic acid
pyruvic acid
nitrogen oxygen trimethylamine
taurine and tryptophan were efficacy of hemostasis biomarkers in charred Zingiberis Rhizoma Recens. Conclusion: Metabolomics can reflect the whole status of metabolism in biosystems
it can be treated as a potential powerful approach in pharmacological studies for different processed products and investigation of essence of syndrome in traditional Chinese medicine.
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