Investigation of Extraction Process and Antioxidation of Total Flavonoids from

GAN Xiu-hai; LIANG Zhi-yuan; ZHAO Chao

doi:10.13422/j.cnki.syfjx.2015160010

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Investigation of Extraction Process and Antioxidation of Total Flavonoids from

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- Investigation of Extraction Process and Antioxidation of Total Flavonoids from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 16, Pages: 10-13(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015160010
  CLC： R285;R284.2
- Published：2015
- 稿件说明：
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GAN Xiu-hai, LIANG Zhi-yuan, ZHAO Chao. Investigation of Extraction Process and Antioxidation of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 10-13.
DOI：

GAN Xiu-hai, LIANG Zhi-yuan, ZHAO Chao. Investigation of Extraction Process and Antioxidation of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 10-13. DOI： 10.13422/j.cnki.syfjx.2015160010.

摘要

目的: 测定不同产地冷水花中总黄酮的含量并评价其抗氧化活性。方法: 在单因素试验基础上

以总黄酮提取量为指标

选择料液比、提取温度、提取时间为考察因素

采用响应面法优化冷水花总黄酮的提取工艺

测定不同产地冷水花样品中总黄酮的含量并考察其抗氧化活性。结果: 最佳提取工艺为料液比1 :19

提取温度71 ℃

提取时间126 min。冷水花总黄酮提取量7.08 mg ·g-1;不同产地样品中总黄酮质量分数4.35~7.10 mg ·g-1

具有明显的地域差异性。冷水花总黄酮对1

1-二苯基苦基苯肼和2

2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基的清除能力与维生素C相当。结论: 不同产地冷水花中总黄酮具有较好的抗氧化能力

且该能力与总黄酮含量具有明显量效关系。

Abstract

Objective: To determine the content of total flavonoids from Pilea aquarum and evaluate its antioxidative activity. Method: With the content of total flavonoids as an indicator

based on single factor experiments

response surface methodology was adopted to optimize extraction process by taking liquid-solid ratio

extraction temperature and time as factors.The content of total flavonoids in P. aquarum from different places were determined

then antioxidation of total flavonoids were evaluated against DPPH and ABTS free radical. Result: Optimum coditions were as follows:liquid-solid ratio of 1 : 19

extraction temperature at 71 ℃ and extraction time of 126 min.Yield of total flavonoids was as high as 7.08 mg · g-1 under these conditions.The content of total flavonoids in P. aquarumfrom different places was from 4.35 to 7.10 mg · g-1 with obvious regional differences.Scavenging capacity of total flavonoids were as same as vitamin C against DPPH and ABTS free radical. Conclusion: There is obvious difference of total flavonoids content in P. aquarum from different places

and total flavonoids has good antioxidative activity in a dose-dependent manner.

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Related Author

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Related Institution

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

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China Academy of Chinese Medical Sciences

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