Content Determination of Five Components in Qigu Capsules by HPLC

YANG Cheng-zi; FAN Xing; BAO Xia-ping; NAN Shu-hua; JIANG Zhi-hong

doi:10.13422/j.cnki.syfjx.2015160060

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Content Determination of Five Components in Qigu Capsules by HPLC

更新时间：2024-01-04

- Content Determination of Five Components in Qigu Capsules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 16, Pages: 60-63(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015160060
  CLC： O657.72;R286.0
- Published：2015
- 稿件说明：
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YANG Cheng-zi, FAN Xing, BAO Xia-ping, et al. Content Determination of Five Components in Qigu Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 60-63.
DOI：

YANG Cheng-zi, FAN Xing, BAO Xia-ping, et al. Content Determination of Five Components in Qigu Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 60-63. DOI： 10.13422/j.cnki.syfjx.2015160060.

摘要

目的: 建立芪骨胶囊的多成分指标定量方法。方法: 采用高效液相色谱法同时测定芪骨胶囊中松果菊苷、朝藿定B

朝藿定C

淫羊藿苷和柚皮苷的含量

Platisil-C18色谱柱(4.6 mm×250 mm

5 μm)

以甲醇-乙腈-水为流动相进行梯度洗脱

流速1 mL ·min-1

检测波长270 nm

柱温 30 ℃。结果: 松果菊苷

柚皮苷

朝藿定B

朝藿定C

淫羊藿苷分别在91～3 094 ng (r=0.999 8)

19～646 ng(r=0.999 7)

11～374 ng(r=0.999 5)

25～850 ng(r=0.999 5)

29～986 ng(r=0.999 6)与峰面积呈良好的线性关系;平均回收率分别为98.9%(RSD 1.2%)

99.1%(RSD 1.3%)

99.1%(RSD 1.5%)

99.4%(RSD 1.5%)

99.3%(RSD 1.5%)。结论: 所建立的方法简便、准确、重复性好

可用于芪骨胶囊的质量控制。

Abstract

Objective: To establish the multi-index quantitative method for Qigu capsules. Method: The contents of echinacoside

naringin

epimedin B

epimedin C and icariin were determined simultaneously by HPLC. Platisil-C18column (4.6 mm×250 mm

5 μm) was used and eluted with the mobile phase of acetonitrile-methanol-water in a gradient mode at the flow rate of 1 mL · min-1. The detection wavelength was 270 nm

and the column temperature was 30 ℃. Result: Echinacoside

naringin

epimedin B

epimedin C

icariin showed a good linear relationship in the ranges of 91-3 094 ng(r=0.999 8)

19-646 ng(r=0.999 7)

11-374 ng(r=0.999 5)

25-850 ng(r=0.999 5)

29-986 ng(r=0.999 6)

respectively. The average recoveries were 98.9% (RSD 1.2%)

99.1% (RSD 1.3%)

99.1% (RSD 1.5%)

99.4% (RSD 1.5%)

99.3% (RSD 1.5%)

respectively. Conclusion: This method is simple

accurate and highly reproducible

and so can be used to control the quality of Qigu capsules.

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