Objective: To establish an HPLC method for simultaneously determining chlorogenic acid

geniposide

baicalin

baicalein and wogonin in Huangzhihua oral liquid (Lonicerae Japonicac Flos

Gardeniae Fructus

Scutellariae Radix

et al). Method: The determination was performed by HPLC on Agilent SB-C18 column (4.6 mm×250 mm

5 μm) by gradient elution at the flow rate of 1.0 mL · min-1. The mobile phase was acetonitrile (solvent A)-0.1% phosphoric acid (solvent B). The detection wavelength was respectively set at 327 nm in the range of 0-13 min

238 nm in the range of 13-15 min and 280 nm in the range of 15-45 min. Result: According to the regression equation

the linear range for chlorogenic acid was 4.096×10-3-1.228 8×10-1μg(r=0.999 3);the linear range for geniposide was 4.204×10-3-1.261 2×10-1μg(r=0.999 9);the linear range for baicalin was 0.117 5-3.525 μg(r=0.999 9); the linear range for baicalein was 4.152×10-3-1.245 6×10-1μg (r=0.999 9);and the linear range for wogonin was 0.425 6×10-3-1.276 8×10-2μg(r=0.999 4). The average recoveries of the above five components were 99.31%(RSD 0.8%);99.62%(RSD 0.5%);99.94%(RSD 1.3%);99.64%(RSD 0.8%);100.1%(RSD 1.2%) respectively. Conclusion: This method is simple

accurate and highly reproducible and so can be applied in the quality control of Huangzhihua oral liquid