XIAO Fu-wen, HU Qiao-hong, LIU Chun-ping, et al. Effect of Micromolecular Hydrophilic Extract from Vingar-baked Bupleuri Radix on Pharmacokinetics of Methotrexate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 76-78.
DOI:
XIAO Fu-wen, HU Qiao-hong, LIU Chun-ping, et al. Effect of Micromolecular Hydrophilic Extract from Vingar-baked Bupleuri Radix on Pharmacokinetics of Methotrexate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 76-78. DOI: 10.13422/j.cnki.syfjx.2015160076.
Effect of Micromolecular Hydrophilic Extract from Vingar-baked Bupleuri Radix on Pharmacokinetics of Methotrexate
Objective: To study the effect of micromolecular hydropilic extract (MHE
a P-glycoprotein inhibitor) from vingar-baked Bupleuri Radix on pharmacokinetics of methotrexate. Method: Sprague-Dawley male rats were orally administrated with methotrexate or that one combined with MHE respectively.Blood samples were collected from their eyes at different time point
treated with 20% perchloric acid protein precipitation method and then analyzed by HPLC.Pharmacokinetic parameters were evaluated by non-compartment model. Result: Compared with the group using methotrexate alone
AUC0-t
AUC0-∞
MRT0-t
t1/2of the one combined with MHE increased by 16.6%
33.8%
26.1%
120%
respectively;while CL decreased by 20.3%
but with no significant difference between their pharmacokinetic parameters. Conclusion: MHE can enhance bioavailability of methotrexate
and attention should be paid to toxic and side effects of their combined administration.
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