Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collagen-induced Rat Arthritis and Inflammatory Cytokines in Serum
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Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collagen-induced Rat Arthritis and Inflammatory Cytokines in Serum
Chinese Journal of Experimental Traditional Medical FormulaeVol. 21, Issue 16, Pages: 97-100(2015)
LI Jing, WANG Meng, GUO Chen, et al. Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collagen-induced Rat Arthritis and Inflammatory Cytokines in Serum[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 97-100.
DOI:
LI Jing, WANG Meng, GUO Chen, et al. Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collagen-induced Rat Arthritis and Inflammatory Cytokines in Serum[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 97-100. DOI: 10.13422/j.cnki.syfjx.2015160097.
Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collagen-induced Rat Arthritis and Inflammatory Cytokines in Serum
0.486 g ·kg-1)、雷公藤多苷组(1.5 mg ·kg-1)和地塞米松组(10.8 mg ·kg-1)分别ig给药
连续给药21 d。观察不同剂量对CIA大鼠关节炎指数、足跖肿胀度的影响
同时测定血清中白介素(IL)-1
IL-1β
IL-6和肿瘤坏死因子-α(TNF-α)水平
以及观察踝关节组织病理变化。 结果: 与正常组比较
模型组关节炎指数和足跖肿胀度显著升高
血清IL-1
IL-1β
IL-6和TNF-α的水平显著升高(P <0.01);与模型组比较
防己水煎液中总生物碱高、中、低剂量组能够降低关节炎指数和足跖肿胀度
明显降低血清中IL-1
IL-1β
IL-6和TNF-α的水平(P <0.01)
并且减轻滑膜组织的病理损伤。 结论: 防己水煎液总生物碱对于类风湿关节炎有一定的治疗作用
可明显降低血清中炎性因子水平。
Abstract
Objective: To study effect of total alkaloids in water extraction from Stephaniae Tetrandrae Radix S.Moore(STRM) on the collagen-induced arthritis(CIA) in order to further explore the anti-rheumatism effects of alkaloidthe water-soluble alkaloids with big polarity. Method: The 732 type cation resin was used to enrich total alkaloid in water extraction from STRM. Rat CIA model was established by injection of bovine Ⅱ collagen type on toes
tail and backside in rats. After 7 days same injection was performed. Alkaloids high
middle
low dose(0.162
0.324
0.486 g · kg-1)
Tripterygium wilfordii glycosides (1.5 mg ·kg-1) and dexamethasone (10.8 mg · kg-1) were administrated for 21 days. The arthritis index(AI) and toes swelling degree were observed
the level of interleukin-1(IL-1)
interleukin-1β(IL-1β)
interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in serum were assayed and the ankle tissue pathological changes were observed. Result: Compared with normal group
AI in model group and toes swelling degree significantly increased(P <0.01).Compared with model group
high
middle
low dose group of total alkaloid in water extraction from STRM could improve AI and toes swelling degree
and significantly decrease the level of IL-1
IL-1β
IL-6 and TNF-α (P <0.01) in serum
and reduce the pathological synovial tissue damage. Conclusion: Total alkaloid in water extraction from STRM has certain therapeutic effect on rheumatoid arthritis
and can significantly decrease the level of inflammatory factors.