WANG Yue, WANG Tao, WU Jian-xia, et al. Anti-diabetic Effect and Mechanism of Components in Jinqi Jiangtang Tablet [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 105-109.
DOI:
WANG Yue, WANG Tao, WU Jian-xia, et al. Anti-diabetic Effect and Mechanism of Components in Jinqi Jiangtang Tablet [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 105-109. DOI: 10.13422/j.cnki.syfjx.2015160105.
Anti-diabetic Effect and Mechanism of Components in Jinqi Jiangtang Tablet
Objective: To explore the anti-diabetic effect and mechanism of components in Jinqi Jiangtang tablet in vitro. Method: A systematic anti-diabetic activity evaluation screening model in vitro was established by scavenging DPPH free radicals
reactive oxygen free radicals
and inhibiting advanced glycation end products formation
alpha-glucosidase and aldose reductase
lipase
NO production from LPS-activated mice macrophages
in order to detect the effect of four standard components—total flavonoids of Astragalus
total saponins of Astragalus
total organic acids of Honeysuckle
Berberine alkaloids in Jinqi Jiangtang tablet on the above mechanisms. Result: Total flavonoids of Astragalus
total organic acids of Honeysuckle
Berberine alkaloids showed scavenging activity on DPPH free radicals and reactive oxygen free radicals;all components showed no inhibitory activity on advanced glycation end products formation;total flavonoids of astragalus
total organic acids of Honeysuckle showed inhibitory activity of alpha-glucosidase;total flavonoids of Astragalus
total saponins of Astragalus
Total organic acids of Honeysuckle showed inhibitory activity of aldose reductase;total flavonoids of Astragalus
total saponins of Astragalus
total organic acids of Honeysuckle showed inhibitory activity of lipase;total saponins of Astragalus
Berberine alkaloids showed inhibitory effects of NO production from LPS-induced mice macrophages. Conclusion: Jinqi Jiangtang tablet has multi-component and multi-target synergistic anti-diabetic effect and achieves the mechanisms by inhibiting alpha-glucosidase
inhibiting lipase
scavenging free radicals
inhibiting aldose reductase and inhibiting the release of NO.