Effect of Buyang Huanwu Tang on Rho Kinase, PAl-1 and eNOS mRNA Expressions in Atherosclerosis Model Aorta

ZHANG Hong-zhen; LI Li; JIAO Rui; WANG Jing; GUO Chun-lan; ZHANG Ying; QIAN Yan

doi:10.13422/j.cnki.syfjx.2015160110

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Effect of Buyang Huanwu Tang on Rho Kinase, PAl-1 and eNOS mRNA Expressions in Atherosclerosis Model Aorta

更新时间：2024-01-04

- Effect of Buyang Huanwu Tang on Rho Kinase, PAl-1 and eNOS mRNA Expressions in Atherosclerosis Model Aorta
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 16, Pages: 110-114(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015160110
  CLC： R285.5
- Published：2015
- 稿件说明：
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ZHANG Hong-zhen, LI Li, JIAO Rui, et al. Effect of Buyang Huanwu Tang on Rho Kinase, PAl-1 and eNOS mRNA Expressions in Atherosclerosis Model Aorta[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 110-114.
DOI：

ZHANG Hong-zhen, LI Li, JIAO Rui, et al. Effect of Buyang Huanwu Tang on Rho Kinase, PAl-1 and eNOS mRNA Expressions in Atherosclerosis Model Aorta[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 110-114. DOI： 10.13422/j.cnki.syfjx.2015160110.

摘要

目的: 通过运用益气活血法的代表方剂补阳还五汤对动脉粥样硬化模型的干预

观察主动脉组织Rho激酶

纤溶酶原激活物抑制物-1(PAI-1)及内皮型一氧化氮合酶(eNOS) mRNA的表达、血脂等指标的变化

揭示该方药抗动脉粥样硬化作用的新靶点和新途径。方法: 60只大鼠随机分为正常组

模型组

补阳还五汤低、高剂量组(10

20 g ·kg-1)

辛伐他汀组(0.6 mg ·kg-1)

补阳还五汤预防组(10 g ·kg-1)

除正常组外

其余各组维生素D3加高脂饮食诱导大鼠动脉粥样硬化模型

分别ig给予相应药物

补阳还五汤预防组给药的同时造模

造模成功后干预28 d

检测主动脉Rho激酶

PAl-1及eNOS mRNA表达

血脂水平总胆固醇(TC)

甘油三酯(TG)

高密度脂蛋白(HDL)

低密度脂蛋白(LDL)和氧化型低密度脂蛋白(ox-LDL)。结果: 与正常组比较

模型组大鼠Rho激酶

PAl-1 mRNA表达量水平明显升高

eNOS mRNA表达量水平明显降低及血脂TC

LDL

ox-LDL水平明显升高(P <0.01)

HDL水平明显降低

均具有明显统计学差异(P <0.01)

补阳还五汤低、高剂量治疗组、辛伐他汀组、补阳还五汤预防组明显降低大鼠Rho激酶

PAl-1 mRNA表达量水平

明显升高eNOS mRNA表达量水平

明显降低血脂TC

LDL

ox-LDL水平

明显升高HDL水平

均具有明显统计学差异(P <0.05

P <0.01)。结论: 补阳还五汤可以下调Rho激酶

PAl-1 mRNA的表达

同时

上调eNOS mRNA的表达水平、降低血脂

具有抗动脉粥样硬化作用

抑制Rho激酶mRNA

PAl-1 mRNA表达及上调eNOS mRNA表达水平可能是其作用机制之一。

Abstract

Objective: To study the effect of representative Qi-tonifying and blood-activating formulae Buyang Huanwu Tang (BYHWT) on the atherosclerosis model

in order to observe the aorta Rho kinase

PAl-1 and eNOS mRNA expressions and blood lipid indicator and reveal new targets and new ways of the formulae in inhibiting atherosclerosis. Method: Totally 60 rats were randomly divided into six groups:the normal control group

the model group

BYHWT low and high dose of groups (10

20 g · kg-1)

the Simvastatin group (0.6 mg · kg-1) and the BYHWT prevention group (10 g · kg-1). Except for the normal group

the other groups were fed with VD3 and high fat diet to induce atherosclerosis and given corresponding drugs. The BYHWT prevention group was administered with drugs during the modeling. After the successful modeling and 28-day intervention

the expression level of Rho kinase

plasma tissue plasminogen activator inhibitor 1(PAl-1)

endothelial nitric oxide synthase(eNOS) mRNA expression

total cholesterol(TC)

triglyceride(TG)

high density lipoprotein cholesterin(HDL-C)

low density lipoprotein cholesterin(LDL-C) and oxidized low-density lipoprotein(ox-LDL) in atherosclerosis aorta were detected. Result: Compared with the normal group

the model group showed significantly higher Rho kinase

PAl-1 mRNA expression

liquid TC

HDL-C

ox-LDL and lower eNOS mRNA expression and LDL-C

all with significant statistical differences (P <0.01);BYHWT low and high dose groups

the Simvastatin group and the BYHWT prevention group showed notably lower Rho kinase

PAl-1 mRNA expression

lipid TC

HDL-C

ox-LDL and higher eNOS mRNA expression

LDL-C

with significant statistical differences (P <0.05

P <0.01). Conclusion: BYHWT has an effect in down-regulating the Rho kinase and PAl-1 mRNA expression

up-regulating the eNOS mRNA expression and blood lipid

resisting atherosclerosis

inhibiting the Rho kinase and PAl-1 mRNA expression and increasing the Rho kinase and eNOS mRNA expression

which may be related to its mechanisms.

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