Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat

JIA Mei; ZHENG Chuan-zhu; ZHANG Li; DING An-wei

doi:10.13422/j.cnki.syfjx.2015160119

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Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat

更新时间：2024-01-04

- Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 16, Pages: 119-123(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015160119
  CLC： R285.5
- Published：2015
- 稿件说明：
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JIA Mei, ZHENG Chuan-zhu, ZHANG Li, et al. Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 119-123.
DOI：

JIA Mei, ZHENG Chuan-zhu, ZHANG Li, et al. Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(16): 119-123. DOI： 10.13422/j.cnki.syfjx.2015160119.

摘要

目的: 比较四物汤不同溶剂提取部位对急性血瘀模型大鼠血液流变学、凝血功能和血小板系统的影响

筛选四物汤活血祛瘀有效部位。方法: 四物汤经水煎煮后

采用系统溶剂提取法分别得到4个溶剂提取部位。雌性SD大鼠80只分为8组

每组10只

分别为正常组

模型组

复方丹参滴丸组(0.15 g ·kg-1)

四物汤组(7 g ·kg-1)

石油醚提取液组(7 g ·kg-1)

乙酸乙酯提取液组(7 g ·kg-1)

正丁醇提取液组(7 g ·kg-1)

水提取液组(7 g ·kg-1)。除正常组外

其余各组采用ih盐酸肾上腺素加冰水浴复制大鼠急性血瘀模型

比较四物汤不同提取部位给药后大鼠血液流变学

凝血酶原时间(PT)

凝血酶时间(TT)

活化部分凝血活酶时间(APTT)

血浆纤维蛋白原(FIB)

血小板聚集率

血浆中血栓素B2(TXB2)和6-酮-前列腺素F1a(6-keto-PGFla)含量的差异。结果: 与正常组比较

模型组各切变率全血黏度、血浆黏度明显升高

APTT明显缩短

FIB含量明显升高

血小板聚集率明显升高

血浆TXB2含量明显升高

6-keto-PGFla的含量明显降低(P <0.01);与模型组比较

四物汤乙酸乙酯组明显降低大鼠各切变率全血黏度、血浆黏度

明显延长TT

APTT

明显降低FIB含量、血小板聚集率、血浆TXB2的含量

明显升高6-keto-PGFla的含量(P <0.01);石油醚组可明显降低高切变率下全血黏度(P <0.05)

降低TXB2含量、升高6-keto-PGFla的含量(P <0.01);正丁醇组、水提组可明显降低TXB2含量、升高6-keto-PGFla含量(P <0.01)。结论: 乙酸乙酯部位是四物汤活血祛瘀的最有效部位。

Abstract

Objective: To screen active fractions of Siwu Tang for activating blood and removing stasis by comparing their effects on the blood rheology

function of blood coagulation and platelet system of the acute model rats. Method: After Siwu Tang was boiled with water

the system solvent extraction method was adopted to extract four fractions respectively. Totally 80 female SD rats were divided into eight groups:the control group

the model group

the Compound Danshen dropping pill group (0.15 g · kg-1)

the Siwu Tang group (7 g · kg-1)

the Petroleum ether extract group (7 g · kg-1)

the Ethyl acetate extract group (7 g · kg-1)

the N-butyl alcohol extract group (7 g · kg-1) and the water extract group (7 g · kg-1). Except for the normal group

the ice water bath and subcutaneous injection with adrenaline hydrochloride were applied in all of the rest groups to establish the acute blood stasis rat model

in order to compare different extracted fractions in the hemorheology of rats after the administration

prothrombin time(PT)

thrombin time(TT)

activate parts thrombin time(APTT)

fibrinogen(FIB)

platelet aggregation rate

plasma thrombox-ane B2(TXB2) and 6-keto-prostaglandin Fla(6-keto-PGFla). Result: Compared with the normal group

the model group showed significant decreases in the shear rate of whole blood viscosity

plasma viscosity

APTT and 6-keto-PGFla and significant increases in FIB

platelet aggregation rate

plasma TXB2.Compared with the normal group

ethyl acetate fractions of Siwu Tang can significantly reduce the shear rate of whole blood viscosity

plasma viscosity

significantly extend TT

APTT

significantly reduce FIB

platelet aggregation rate

plasma TXB2

elevate 6-keto-PGFla;the Petroleum ether group can notably decrease the whole blood viscosity at a high shear rate (P <0.05)

reduce TXB2 content

increase 6-keto-PGFla content (P <0.01);N-butyl alcohol fractions and water extracts can obviously reduce TXB2 and increase 6-keto-PGFla (P <0.01). Conclusion: Ethyl acetate fractions of Siwu Tang have the most effective effect in removing blood stasis.

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