Optimization of Extraction Process of Volatile Oil from  Leaves with Orthogonal Design and Analysis of Its Chemical Components

YANG Guang-an; HUANG Qian; LIN Bing; ZHOU Ying; YANG Shi-lin

doi:10.13422/j.cnki.syfjx.2015170009

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Optimization of Extraction Process of Volatile Oil from Leaves with Orthogonal Design and Analysis of Its Chemical Components

更新时间：2024-01-04

- Optimization of Extraction Process of Volatile Oil from Leaves with Orthogonal Design and Analysis of Its Chemical Components
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 17, Pages: 9-13(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015170009
  CLC： R284.2
- Published：2015
- 稿件说明：
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YANG Guang-an, HUANG Qian, LIN Bing, et al. Optimization of Extraction Process of Volatile Oil from Leaves with Orthogonal Design and Analysis of Its Chemical Components[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 9-13.
DOI：

YANG Guang-an, HUANG Qian, LIN Bing, et al. Optimization of Extraction Process of Volatile Oil from Leaves with Orthogonal Design and Analysis of Its Chemical Components[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 9-13. DOI： 10.13422/j.cnki.syfjx.2015170009.

摘要

目的: 优选海通叶挥发油的提取工艺并分析其化学成分

为该药材的资源开发提供参考。方法: 在单因素试验基础上

采用正交试验考察加水量、浸泡时间、蒸馏时间和超声时间对提取工艺的影响。利用气相色谱-质谱联用仪分析海通叶挥发油的成分

通过峰面积归一化法测定各成分的相对质量分数。结果: 最佳提取工艺为加12倍量水浸泡12 h

蒸馏提取10 h

超声80 min

挥发油得率0.332%。共分离出59化合物

鉴定了其中53个化学成分

占总挥发油的95.14%。相对质量分数>5%的化学成分有1-辛烯-3-醇(19.020%)

芳樟醇(12.981%)

植醇(11.768%)

己烯醛(9.911%)及α-蒎烯(6.681%)。结论: 优选的海通叶挥发油提取工艺稳定合理

挥发油的提取效率较高。海通叶挥发油主要化学成分为脂肪烃类和萜类及其衍生物。

Abstract

Objective: To optimize extraction process of volatile oil from Clerodendrum mandarinorum leaves

and analyze its chemical components. Method: On the basis of single factor tests

the amount of water

soaking time

distillation time and ultrasonic time was optimized by orthogonal test.Chemical components in volatile oil were analyzed by GC-MS

the relative content of each component was determined by peak area normalization method. Result: Optimum extraction technology conditions were as follows:soaked in 12 times the amount of water for 12 hours

distilled for 10 hours

ultrasonic time of 80 min.Yield of volatile oil was 0.332%.Fifty-nine compounds were separated and fifty-three of them were identified accounting for 95.14% of the total content.Chemical components were 1-octen-3-ol(19.020%)

linalool(12.981%)

phytol(11.768%)

2-hexenal(9.911%)

alpha-pinene(6.681%)

whose relative mass fractions were greater than 5%. Conclusion: Orthogonal test can accurately select extraction process of volatile oil from C. mandarinorum leaves

this process is reasonable with high efficiency.Main components of volatile oil are aliphatic hydrocarbons

terpenes and their derivatives.

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Related Institution

School of Pharmacy,Nanjing University of Traditional Chinese Medicine

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Dongzhimen Hospital， Beijing University of Chinese Medicine

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