Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC

DONG Yun-zhuo; SONG Zhi-qian; LIU Zhen-li; LIU Yuan-yan; DU Zhi-yong; NING Zhang-chi; WANG Chun

doi:10.13422/j.cnki.syfjx.2015170028

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Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 17, Pages: 28-31(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015170028
  CLC： O657.72;R284.1
- Published：2015
- 稿件说明：
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DONG Yun-zhuo, SONG Zhi-qian, LIU Zhen-li, et al. Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 28-31.
DOI：

DONG Yun-zhuo, SONG Zhi-qian, LIU Zhen-li, et al. Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 28-31. DOI： 10.13422/j.cnki.syfjx.2015170028.

摘要

目的: 建立多波长同时测定桂枝芍药知母汤中芒果苷、芍药苷、升麻素苷、甘草苷、5-O-甲基维斯阿米醇苷、异甘草苷和甘草酸铵含量的方法。方法: 采用HPLC法

Zorbax SB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.01%甲酸水梯度洗脱

流速1.0 mL·min-1

检测波长250 nm(芒果苷)

237 nm(芍药苷、升麻素苷、甘草酸铵)

280 nm(甘草苷

5-O-甲基维斯阿米醇苷)

355 nm(异甘草苷)

柱温30 ℃。结果: 芒果苷、芍药苷、升麻素苷、甘草苷和5-O-甲基维斯阿米醇苷、异甘草苷、甘草酸铵进样量分别在0.264 8~2.648(r=0.999 9)

0.81~8.10(r=0.999 8)

0.230 4~2.304(r=0.999 9)

0.173~1.73(r=0.999 9)

0.115~1.15(r=0.999 8)

0.028 4~0.284(r=0.999 8)

0.252 4~2.524 μg(r=0.999 9)与色谱峰面积呈良好的线性关系;加样回收率均在97.41%~102.0%

RSD均<3.0%。结论: 该方法简单、准确

重复性好

为桂枝芍药知母汤物质基础研究提供了基础。

Abstract

Objective: To establish an HPLC multi-wavelength method for determination of mangiferin

paeoniflorin

prim-O-glucosylcimifugin

iquiritin

5-O-methylvisamminol

isoliquiritoside and glycyrrhizic acid in Guizhi Shaoyao Zhimu Tang. Method: Zorbax SB-C18 column (4.6 mm×250 mm

5 μm) was eluted with the mobile phase of acetonitrile-0.01% formic acid water in a gradient mode. The flow rate was 1 mL·min-1.The detection wavelength was set at 250 nm (mangiferin)

237 nm (paeoniflorin

prim-O-glucosylcimifugin

glycyrrhizic acid)

280 nm (iquiritin

4'-O-β-glucopyranosyl-5-O-methylvisamminol)

and 355 nm (isoliquiritoside). The column temperature was maintained at 30 ℃. Result: Mangiferin

paeoniflorin

prim-O-glucosylcimifugin

iquiritin

5-O-methylvisamminol

isoliquiritoside and glycyrrhizic acid had a good linearity within the ranges of 0.264 8-2.648(r=0.999 9)

0.81-8.10(r=0.999 8)

0.230 4-2.304(r=0.999 9)

0.173-1.73(r=0.999 9)

0.115-1.15(r=0.999 8)

0.028 4-0.284(r=0.999 8)

0.252 4-2.524 μg(r=0.999 9)

with the recoveries between 97.41%-102.0% and RSD<3.0%. Conclusion: This method is simple

accurate and highly reproducible and can provide basis for studying material base of Guizhi Shaoyao Zhimu Tang.

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