LIANG Cong-lian, LIU Hong-yan, CUI Yuan, et al. Chromatography-efficacy Relation Between HPLC Fingerprints of Lonicerae Japonicae Flos and Its Effect in Scavenging DPPH Free Radicals[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 44-46.
DOI:
LIANG Cong-lian, LIU Hong-yan, CUI Yuan, et al. Chromatography-efficacy Relation Between HPLC Fingerprints of Lonicerae Japonicae Flos and Its Effect in Scavenging DPPH Free Radicals[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 44-46. DOI: 10.13422/j.cnki.syfjx.2015170044.
Chromatography-efficacy Relation Between HPLC Fingerprints of Lonicerae Japonicae Flos and Its Effect in Scavenging DPPH Free Radicals
Objective: To investigate the active components in Lonicerae Japonicae Flos for scavenging DPPH free radicals. Method: The HPLC fingerprints were developed and merged. Meanwhile
the DPPH radical scavenging equation was established. The bivariate correlation analysis was applied to correlate the scavenging DPPH Free radical parameter IC50 and the standardized peak area ratio of common peaks of HPLC fingerprints. Result: The changes in the contents of common peaks 4 (chlorogenic acid)
10 (isochlorogenic acid B) and 12 (isochlorogenic acid C) show significant negative correlation with DPPH free radical scavenging activity. Conclusion: The chromatography-efficacy relation between the HPLC fingerprints and the DPPH free radical scavenging activity provides a certain reference value for the basic study on medicinal material base of Lonicerae Japonicae Flos.