GC-MS Fingerprint and Antimicrobial Activities of Volatile Oils from

GAN Xiu-hai; LIANG Zhi-yuan; ZHOU Xin; ZHAO Chao; JIANG Jin-zhong

doi:10.13422/j.cnki.syfjx.2015170063

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GC-MS Fingerprint and Antimicrobial Activities of Volatile Oils from

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- GC-MS Fingerprint and Antimicrobial Activities of Volatile Oils from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 17, Pages: 63-66(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015170063
  CLC： O657.63;R284.1
- Published：2015
- 稿件说明：
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GAN Xiu-hai, LIANG Zhi-yuan, ZHOU Xin, et al. GC-MS Fingerprint and Antimicrobial Activities of Volatile Oils from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 63-66.
DOI：

GAN Xiu-hai, LIANG Zhi-yuan, ZHOU Xin, et al. GC-MS Fingerprint and Antimicrobial Activities of Volatile Oils from [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 63-66. DOI： 10.13422/j.cnki.syfjx.2015170063.

摘要

目的: 建立冷水花挥发油GC-MS指纹图谱

测定其抗菌活性。方法: 利用GC-MS法分析10批次冷水花挥发油样品

采用“中药色谱指纹图谱计算机相似性评价系统”软件进行模式分析及相似度计算

对共有特征峰成分进行检识;采用滤纸片法测试了挥发油的抗菌活性。结果: 建立了冷水花挥发油GC-MS指纹图谱

标定并鉴定了16个共有特征峰;冷水花挥发油对金黄色葡萄球菌、大肠埃希菌和枯草芽孢杆菌具有一定的抑菌活性。结论: 该方法重复性好

所建立的指纹图谱为贵州冷水花挥发油的质量控制提供了有效手段

为冷水花挥发油的利用提供了重要依据。

Abstract

Objective: To establish characteristic fingerprint of volatile oils from Pilea aquarum by gas chromatography-mass spectrometry to determine the antimicrobial activities of volatile oils. Method: Chemical components of volatile oil from ten batches of P.aquarum were analyzed by GC-MS method

the common model and similarity of the TIC profiles were analyzed by “Computer Aided Similarity Evaluation System”

and the components of common characteristic peaks in chromatograms were identified. Antimicrobial activities of volatile oils were tested by filtering paper method. Result: Fingerprint of volatile oils from P.aquarum were established

sixteen characteristic peaks in GC-MS specific chromatogram of volatile oils from P.aquarum were identified and defined. The results also showed that the volatile oils of P.aquarum showed a cetain effect against Staphylococcus aureus

Escherichia coli and Bacillus subtilis. Conclusion: With a good reproducibility

the established characteristic fingerprints of volatile oil from P.aquarum can be used for quality control and provide important basis for application of volatile oils from P.aquarum.

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College of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine

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