Objective: A HPLC method was established for the determination of chlorogenic acid

geniposide

luteolin

baicalin

phillyrin and wogonin in Qingrejiedu capsules. Method: Eclipse XDB column (4.6 mm×150 mm

5 μm) was adopted and eluted in gradient with the mobile phase of acetonitrile-0.4% phosphoric acid solution. The flow rate was 0.80 mL·min-1. The detection wavelength was set at 275 nm. Chlorogenic acid

geniposide

luteolin

baicalin

phillyrin and wogonin could be completely separated from adjacent mass spectra peaks. Result: The HPLC method showed a good linear range of 7.83-313 mg·L-1 (r=0.999 9) for chlorogenic acid

4.30-132 mg·L-1(r=0.999 8) for geniposide

0.836-33.5 mg·L-1(r=0.999 7) for luteolin

4.36-349 mg·L-1 (r=1.000 0) for baicalin

0.731-58.4 mg·L-1(r=0.999 7) for phillyrin and 0.314-12.6 mg·L-1 (r=0.999 7) for wogonin

with the recoveries above 98%. Conclusion: The method is simple

accurate and highly reproducible and so can be used to remove interferences from other components and evaluate the quality control of the preparation.