Objective: To observe the changes in intestinal epithelial microvillus of functional diarrhea spleen deficiency symptom model rats after the treatment with Pixu Ⅳ recipe. Method: Totally 36 male healthy rats were divided into the normal group

the model group

Pixu Ⅳ recipe high

medium and low doses groups (4.6

2.3

1.2 g·kg-1) and the positive Smecta (0.9 g·kg-1) group. The high fat sucrose diets and the water environment small platform standing method were adopted to establish the diarrhea model. After the successful modeling

the rats were orally given drugs for 7 days. The transmission electron microscopy was used to observe the effect of Pixu Ⅳ recipe on the microstructure in intestinal epithelial microvillus. Result: The normal group showed neat and even microvilli. The model group showed small changes in microvilli of the jejunum mucous membrane;significant changes in microvilli of ileum mucous membrane

with uneven microvilli of ileum mucous membrane and low electron density under electron microscope(×25 000)

serious damages in proximal and distal colons

particularly uneven

thin and irregular microvilli and more secreted vesicles under mucous membrane. All of the above phenomena were relieved to varying extent after the intervention with Pixu Ⅳ recipe and positive drugs. The Pixu Ⅳ recipe low dose group showed the most significant relieve

which was close to the normal group;the Pixu Ⅳ recipe high dose group was next to it

whereas the Pixu Ⅳ recipe medium dose group and the positive drug group showed no significant difference. Conclusion: The diarrhea model established with the high fat sucrose diets and the water environment small platform standing method shows no significant morphologic change in epithelial cell microvilli of jejunal mucosa and gradually obvious morphologic changes and damage degree from ileum

proximal colon to distal colon

the changes became more and more serious. Pixu Ⅳrecipe can relive the microstructure changes of the microvilli in the diarrhea rats

which are maked by the high fat sucrose diet and modified multiple platform method. The Pixu Ⅳ recipe low dose group showed the most significant relieve

which was followed by the Pixu Ⅳ recipe high dose group.