Objective: To investigate the preliminary mechanism and manifestations among the anti-drug compatibility of which Pinelliae Rhizoma weakened the anti-inflammatory effects of Aconiti Kusnezoffii Radix. Method: Sixty-four SD rats were randomly divided into model group

prednisone group

Aconiti Kusnezoffii Radix group

the Aconiti Kusnezoffii Radix and Pinelliae Rhizoma compatibility 9:1 group

3:1 group

1:1 group

1:3 group

1:9 group.The prednisone group was given at dose of 20 mg·kg-1 by gavage

the Aconiti Kusnezoffii Radix group was given at dose of 0.6 g·kg-1 by gavage

the Aconiti Kusnezoffii Radix and Pinelliae Rhizoma compatibility 9:1 group

3:1 group

1:1 group

1:3 group

1:9 group were given at dose of 0.67

0.8

1.2

2.4

6.0 g·kg-1by gavage

the model group were treated with the same volume's physiological saline for 7 days.An hour after administration on the 7th day

the egg-white was injected into the rats right paw to establish the egg-white induced rat paw edema model. Rat's tail vein injection of Evans blue (EB)

and determination of tissue EB

serum interleukin-1 (IL-1)

prostaglandin E2(PGE2)

tumor necrosis factor-α (TNF-α) content by ELISA

study the difference of paw swelling degree

vascular permeability and the release of inflammatory mediators between Aconiti Kusnezoffii Radix and different proportions of Pinelliae Rhizoma.By GraphPad Prism 5 software to do a four-parameter fit equation of Hill

and judge the impact indicators which was more closely related to paw swelling. Result: Compared with the model group

Aconiti Kusnezoffii Radix group

Aconiti Kusnezoffii Radix and Pinelliae Rhizoma compatibility group could significantly reduce the extent of rat's paw edema and the EB content in tissue

and the content of IL-1

PGE2

TNF-α in serum.The Aconiti Kusnezoffii Radix group compared with the Aconiti Kusnezoffii Radix and various proportions of Pinelliae Rhizoma compatibility group

Aconiti Kusnezoffii Radix group showed the strongest anti-inflammatory action(P<0.05).The Aconiti Kusnezoffii Radix and Pinelliae Rhizoma compatibility group (9: 1

3: 1

1: 1

1: 3

1: 9) of anti-inflammatory effects was weakened in turn of the increase of the amount of Pinelliae Rhizoma.The content of IL-1

PGE2

TNF-α in serum were in good correlation with the paw swelling

but the content of EB in the tissue was to the opposite. Conclusion: Pinelliae Rhizoma can reduces the anti-inflammatory activity of Aconiti Kusnezoffii Radix

and the inhibitory effect increased with the amount gradually increase of Pinelliae Rhizoma. The content of PGE2 in serum had a better correlation with the degree of swelling

and are directly involved in inflammatory reaction medium

so it can be the indicator of Pinelliae Rhizoma which weakens the anti-inflammatory effect of Aconiti Kusnezoffii Radix.