Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells

ZHU Fu-liang; LIU Jin-yang; HUANG Feng-xiang

doi:10.13422/j.cnki.syfjx.2015170126

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Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells

更新时间：2024-01-04

- Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 17, Pages: 126-128(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015170126
  CLC： R285
- Published：2015
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ZHU Fu-liang, LIU Jin-yang, HUANG Feng-xiang. Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 126-128.
DOI：

ZHU Fu-liang, LIU Jin-yang, HUANG Feng-xiang. Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 126-128. DOI： 10.13422/j.cnki.syfjx.2015170126.

摘要

目的: 初步研究莪术油对骨肉瘤saos-2细胞的作用

并探讨胰岛素样生长因子-1受体(IGF-1R)

蛋白激酶B(Akt)及B细胞淋巴瘤/白血病-2(Bcl-2)的表达情况。方法: 以saos-2细胞为研究对象

采用MTT法观察不同质量浓度30

120 mg·L-1莪术油作用saos-2细胞24

72 h后的增殖影响

流式细胞术观察莪术油(30

120 mg·L-1)诱导saos-2细胞的凋亡

RT-PCR检测IGF-1R mRNA的表达

Western blot检测Akt及Bcl-2的蛋白表达变化。结果: 与空白组比较

莪术油(30

120 mg·L-1)能明显抑制saos-2细胞的增殖

并诱导saos-2细胞凋亡

呈剂量依赖性

明显下调IGF-1R mRNA的表达

明显下调Akt及Bcl-2蛋白的表达(P<0.05

P<0.01)。结论: 莪术油具有抑制saos-2细胞生长和诱导凋亡的作用

其抗肿瘤作用与抑制IGF-1R

Akt及Bcl-2的表达有相关性。

Abstract

Objective: To study the effect of Curcumae Rhizoma oil on human osteosarcoma cell line saos-2

and discuss the change of the expression level of insulin-like growth factor-1 receptor (IGF-1R)

protein kinase B (Akt) and B-cell lymphoma/leukemia-2 (Bcl-2). Method: The effects of Curcumae Rhizoma oil on the proliferation of osteosarcoma cell line saos-2 were detected by MTT assay. Cytometry was used to analyze cell apoptosis; expression of IGF-1R was detected by real-time PCR

and the expression of Akt and Bcl-2 was detected by western-blot. Result: Curcumae Rhizoma oil could obviously inhibit the growth of osteosarcoma cells and induce saos-2 cell apoptosis in a dose dependent manner. The expression of IGF-1R

Akt and Bcl-2 was down regulated in the curcumol-treated cells. Conclusion: Curcumae Rhizoma oil can inhibit proliferation and induce apoptosis of saos-2 cells

and its antitumor effect is associated with suppressing the expression of IGF-1R

Akt and Bcl-2.

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