DU Mao-bo, LIU Shu-zhi, SHEN Shuo, et al. Optimization of Purification Process of Yuanhu Zhitong Soft Capsules by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 13-16.
DOI:
DU Mao-bo, LIU Shu-zhi, SHEN Shuo, et al. Optimization of Purification Process of Yuanhu Zhitong Soft Capsules by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 13-16. DOI: 10.13422/j.cnki.syfjx.2015180013.
Optimization of Purification Process of Yuanhu Zhitong Soft Capsules by Macroporous Resin
Objective: To optimize purification process of Yuanhu Zhitong soft capsules about removing tannins and enriching alkaloids. Method: Gallic acid was indicators to purify tannins
tetrahydropalmatine was indicators to purify alkaloids.Single factor test was adopted to optimize purification process of removing gallic acid and enriching tetrahydropalmatine in Yuanhu Zhitong soft capsules. Result: AB-8 macroporous resin was selected
its best process of enriching tetrahydropalmatine was:the concentration of sample solution of 0.28 g· mL-1
diameter height ratio of 1:10
the largest adsorption capacity of 1.366 mg for per gram of resin
eluent of 95% ethanol
elution flow rate of 2 BV· h-1
eluent amount of 5 BV.Under these conditions
adsorption rate was 84.7%
elution ratio was 85.0%.Purity of total alkaloids in the final product was up to 3.63%. Conclusion: AB-8 resin can be used to remove tannins and enrich alkaloids
this study provides a reference for improving clinical efficacy and patient compliance of Yuanhu Zhitong soft capsules.
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