WANG Jian-cheng. Optimization of Purification Process of Qiangzhi Yishen Capsules with Macroporous Absorptive Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 17-20.
DOI:
WANG Jian-cheng. Optimization of Purification Process of Qiangzhi Yishen Capsules with Macroporous Absorptive Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 17-20. DOI: 10.13422/j.cnki.syfjx.2015180017.
Optimization of Purification Process of Qiangzhi Yishen Capsules with Macroporous Absorptive Resin
Objective: To optimize purification process of Qiangzhi Yishen capsules with macroporous resin. Method: Absorption ratio and elution rate of tenuifolin
paeoniflorin
glycyrrhizic acid and liquiritin were taken as indexes to screen out types of macroporous resins.Effects of the concentration of solution
diameter-height ratio and eluent volume on purification process of Qiangzhi Yishen capsules were investigated by single factor tests. Result: HPD-100 macroporous resin was selected
optimum purification process was as following:the mass concentration of sample loading solution 0.1 g· mL-1
resin-material drug (3:1)
diameter-height ratio of resin 1:7.5
sample loading speed 3 BV· h-1
decontaminated with 4 BV of water
and then eluted with 5 BV of 70% ethanol
elution speed of 3 BV· h-1.Transport rates of tenuifolin
paeoniflorin
glycyrrhizic acid and liquiritin were 78.55%
88.62%
82.47%
83.15%
with rate of extract of 7.52% and drying time of 0.5 h. Conclusion: This process can effectively remove starch
saccharides and other impurities in water extract
these purified substances is easy to use in preparations.