Objective: To develop a RP-HPLC method for the simultaneous determination of 16 amino acids in Xianling Gubao capsule. Method: With norleucine as the internal standard

hydrolyzed amino acids of Xianling Gubao capsule were derivatized with phenylisothiocyanate (PITC) and determined by RP-HPLC. Hypersil C18 column (4.6 mm×250 mm

5 μm) was eluted with mobile phase A acetonitrile-0.1 mol· L-1 sodium acetate solution-acetic acid (7:93:0.05) and mobile phase B acetonitrile-water (80:20) in a gradient mode at a flow rate of 1 mL· min-1. The detection wavelength was set at 254 nm and the column temperature was 40 ℃. Result: The 16 amino acids were separated within 33 min with a good linearity (r>0.996 8) within the range of the test concentration. The average recoveries ranged between 97.5% and 100.7% and the RSD values were less than 5%. Conclusion: The method is simple

quick

accurate and reliable and can be used for the determination of amino acids in Xianling Gubao capsule.