Effects of Volatile Oil from Cinnamomi Ramulus and Cinnamaldehyde on Cytokines and T Cell Subsets in H1N1-infected Mice

LIU Rong; WU Zhi-qiang; HE Min; QUE Chang-tian; YANG Jing; ZENG Nan

doi:10.13422/j.cnki.syfjx.2015180139

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Effects of Volatile Oil from Cinnamomi Ramulus and Cinnamaldehyde on Cytokines and T Cell Subsets in H1N1-infected Mice

更新时间：2024-01-04

- Effects of Volatile Oil from Cinnamomi Ramulus and Cinnamaldehyde on Cytokines and T Cell Subsets in H1N1-infected Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 18, Pages: 139-143(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015180139
  CLC： R285.5
- Published：2015
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LIU Rong, WU Zhi-qiang, HE Min, et al. Effects of Volatile Oil from Cinnamomi Ramulus and Cinnamaldehyde on Cytokines and T Cell Subsets in H1N1-infected Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 139-143.
DOI：

LIU Rong, WU Zhi-qiang, HE Min, et al. Effects of Volatile Oil from Cinnamomi Ramulus and Cinnamaldehyde on Cytokines and T Cell Subsets in H1N1-infected Mice[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 139-143. DOI： 10.13422/j.cnki.syfjx.2015180139.

摘要

目的: 研究桂枝挥发油及主要成分桂皮醛抗甲型流感病毒A/PR/8/34(H1N1)作用及其抗病毒作用机制。方法: KM小鼠70只随机分为7组

分别为正常组

模型组

利巴韦林组(0.15 g·kg-1)

桂枝挥发油高、低剂量组(0.348

0.174 mg·kg-1)

桂皮醛高、低剂量组(0.264

0.132 mg·kg-1)

每组10只。将浓度为30 LD50的H1N1病毒液50 μL注入小鼠气管

复制病毒性肺炎模型小鼠

观察连续ig给药5 d对模型小鼠肺组织血凝滴度的抑制作用;ELISA法观察上述组别连续ig给药5 d对模型小鼠血清细胞因子白细胞介素-2(IL-2)

IL-6和肿瘤坏死因子-α(TNF-α)分泌量的影响;流式细胞仪法测定桂枝挥发油0.348 mg·kg-1

桂皮醛0.264 mg·kg-1

正常组

模型组及利巴韦林组连续ig给药5 d对模型小鼠外周血T细胞亚群的影响。结果: 与正常组比较

模型组小鼠肺组织血凝滴度

小鼠血清IL-6

TNF-α含量明显升高

小鼠外周血CD4+/CD8+

小鼠血清IL-2含量明显降低(P<0.05);与模型组比较

桂枝挥发油0.348 mg·kg-1

桂皮醛0.264 mg·kg-1能明显降低模型小鼠肺组织血凝滴度(P<0.05)

桂皮醛0.264 mg·kg-1和桂枝挥发油0.348 mg·kg-1分别显著降低模型小鼠血清IL-6

TNF-α含量(P<0.05)

桂皮醛0.264 mg·kg-1可使模型小鼠外周血CD4+/CD8+明显升高(P<0.05)

各药物对小鼠血清IL-2含量的影响并不明显。结论: 桂枝挥发油、桂皮醛治疗给药能明显降低模型小鼠肺组织血凝滴度

表现出较好的抗流感病毒作用。抗病毒机制与调控模型小鼠血清细胞因子IL-2

IL-6

TNF-α分泌水平及提高外周血T细胞亚群的比例有关。

Abstract

Objective: To study the effect and anti-influenza A virus (H1N1) mechanism of volatile oil from Cinnamomi Ramulus (VOCR) and its major component cinnamaldehyde in vivo. Method: Totally 70 KM mice were divided into seven groups:the control group

the model group

the Ribavirin group (0.15 g· kg-1)

VOCR high and low dose groups (0.348

0.174 mg· kg-1) and cinnamaldehyde high and low dose groups (0.264

0.132 mg· kg-1)

with 10 mice in each group. The model group was intratracheally injected with 50 μL H1N1 liquid to reproduce the H1N1 model and then continuously given drugs for 5 d to observe the inhibitory effect in the hemagglutination titer in lung tissues. The ELISA test was conducted to observe the effect on the secretions of serum interleukin-2(IL-2)

IL-6 and tumor necrosis factor-α(TNF-α). Flow cytometry was used to detect the effect on T cell subsets after the five-day continuous treatment in the VOCR (0.348 mg· kg-1) group

the cinnamaldehyde (0.264 mg· kg-1) group

the control group

the model group and the Ribavirin (0.15 g· kg-1) group. Result: Compared with the normal group

the model group showed significant increases in the hemagglutination titer in lung tissues

mice serum IL-6 and TNF-α but notable decreases in CD4+/CD8+ and IL-2 content (P<0.05). Compared with the normal group

the VOCR (0.348 mg· kg-1) group and the cinnamaldehyde (0.264 mg· kg-1) group showed reduction in the serum levels of IL-6 and TNF-α separately (P<0.05);the Cinnamaldehyde (0.264 mg· kg-1) obviously increased the rate of CD4+ to CD8+ T cells (P<0.05). All groups show no remarkable effect on serum IL-2 content. Conclusion: VOCR and cinnamaldehyde could significantly decrease the hemagglutination titer in lung tissues of H1N1-infected mice and show a better anti-H1N1 effect. The mechanism of anti-influenza A virus effect s correlated with the regulation of the ratio between the secretions of serum cytokines IL-2

IL-6

TNF-α and the increase in T cell subsets of model mice.

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