Comparison Among Different Fraction Formulas of Five Flavonoids in Cellular Anti-inflammation, Immunity and Chondrocyte Proliferation Activity

ZHANG Li-guo; NI Li-jun; ZHAO Li-li; YAN Zhi-hui

doi:10.13422/j.cnki.syfjx.2015180149

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Comparison Among Different Fraction Formulas of Five Flavonoids in Cellular Anti-inflammation, Immunity and Chondrocyte Proliferation Activity

更新时间：2024-01-04

- Comparison Among Different Fraction Formulas of Five Flavonoids in Cellular Anti-inflammation, Immunity and Chondrocyte Proliferation Activity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 18, Pages: 149-154(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015180149
  CLC： R285.5
- Published：2015
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ZHANG Li-guo, NI Li-jun, ZHAO Li-li, et al. Comparison Among Different Fraction Formulas of Five Flavonoids in Cellular Anti-inflammation, Immunity and Chondrocyte Proliferation Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 149-154.
DOI：

ZHANG Li-guo, NI Li-jun, ZHAO Li-li, et al. Comparison Among Different Fraction Formulas of Five Flavonoids in Cellular Anti-inflammation, Immunity and Chondrocyte Proliferation Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(18): 149-154. DOI： 10.13422/j.cnki.syfjx.2015180149.

摘要

目的: 以腰痛宁中生物碱加黄酮有效部位拆方为基础比较不同中药黄酮有效部位对细胞抗炎、免疫及骨细胞修复的影响

为风湿骨病处方候选药物筛选有效部位提供依据。方法: 在腰痛宁马钱子、麻黄生物碱基础上

分别加入50%的甘草黄酮、淫羊藿黄酮、红花黄酮、骨碎补黄酮、桑寄生黄酮以及5种黄酮的等比例混合物

得到6个有效部位组方。采用半数有效浓度(EC50)或半数抑制浓度(IC50)评价各样品对巨噬Ana-1细胞分泌白细胞介素1β(IL-1β)

白细胞介素-6(IL-6)

肿瘤坏死因子-α(TNF-α)炎症因子的促进作用;对脂多糖(LPS)诱导的Ana-1细胞释放前列腺素E2(PGE2)的抑制作用;对IL-1β诱导的软骨细胞增殖的影响。同时采用最小二乘优化方法

计算各样品的EC50或IC50叠加值

根据EC50或IC50叠加值与实验值之间的差异分析各有效部位间的相互作用关系。结果: 骨碎补黄酮为主的组方促进IL-1β分泌的活性最强;红花黄酮为主的组方促进IL-6分泌的活性最强且其他活性仅次于最佳的组方;由马钱子、麻黄生物碱+5种黄酮混合物构成的组方抑制PGE2分泌及促进TNF-α分泌、促进软骨细胞增殖的活性最强

且各模型下该组方中有效部位间有极强的协同作用。促软骨细胞增殖模型下

单一黄酮有效部位与生物碱构成的组方中的有效部位之间均表现出较强的拮抗作用。结论: 不同药材黄酮之间有出色的协同增效作用

红花黄酮、骨碎补黄酮及各药材黄酮混合物具有良好的抗炎、免疫及骨细胞修复等综合药理活性

提示红花黄酮、骨碎补黄酮及不同药材黄酮混合物可作为风湿骨病药物的优选有效部位。

Abstract

Objective: To compare the effect of different flavonoids on a cell anti-inflammation

immunity and chondrocyte proliferation based on the decomposed formula of Yaotongning capsule (YTNC)

in order to provide basis for screening candidate fractions treating rheumatoid bone diseases. Method: Five flavonoids:Glycyrrhiza uralensis (Fgu)

Epimedium sagittatum (Fes)

Carthamus tinctorius (Fct)

Davallia mariesii (Fdm)

Taxillus chinensis (Ftc)

at 50% and their proportional compounds were mixed into alkaloids of Strychnos nux-vomica and Ephedra sinica to obtain six formulas. Their half effective inhibitory concentration (IC50) and half effective concentrations (EC50) were adopted to evaluate the effect of the samples in promoting interleukin-6(IL-6)

IL-1β

tumor necrosis factor-α(TNF-α) inflammatory factor in macrophages Ana-1

inhibiting prostaglamd in E2(PGE2) secretion released from Ana-1 cells induced by LPS and promoting chondrocyte proliferation. The optimization method based on least squares was applied to calculate additive EC50/IC50 value of each sample. The interactions between the effective fractions were analzed by comparing the differences between the additive EC50/IC50 values with the corresponding experimental EC50/IC50 values. Result: The formula mainly consisting of Fdm had the best effect in promoting the secretion of IL-1β. The formula consisting of Fct had the best effect in stimulating IL-6 secretion

and other activities second only to the best formulas. The formula consisting of Strychnos nux-vomica

Ephedra sinica and the five flavonoids had the best effect among the inhibiting PGE2 secretion and promoting TNF-α secretion and chondrocyte proliferation

and a strong synergistic effect among the effective fractions in this formula was observed. In the model for promoting chondrocyte proliferation

there were strong antagonistic effects among effective fractions in the formula consisting of single flavonoid and alkaloid. Conclusion: There is excellent synergistic interaction among different kinds of flavonoids. Fct

Fdm and flavonoid mixtures have good synthetic pharmacological activities in anti-inflammation

immunomodulation and chondrocyte proliferation

suggesting that Fct

Fdm and flavonoid mixtures can be used to optimize candidate effective fractions of flavonoids for traditional Chinese medicines for treating rheumatism bone diseases.

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Related Institution

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The First College of Clinical Medical Seience,Three Gorges University,department of Clinical Laboratory,Yichang Central People’s Hospital

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