Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design

ZHU Ya-nan; LI Ping; WANG Man; WANG Lu-lu; JU Jian-ming; TAO Di-sheng

doi:10.13422/j.cnki.syfjx.2015190006

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Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design

更新时间：2024-01-04

- Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 19, Pages: 6-9(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015190006
  CLC： R283
- Published：2015
- 稿件说明：
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ZHU Ya-nan, LI Ping, WANG Man, et al. Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 6-9.
DOI：

ZHU Ya-nan, LI Ping, WANG Man, et al. Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(19): 6-9. DOI： 10.13422/j.cnki.syfjx.2015190006.

摘要

目的:优选痤疮消凝胶的提取工艺

为该制剂的临床应用提供参考。方法:以大黄游离总蒽醌、栀子苷、咖啡酸含量的综合评分为指标

采用均匀设计法考察乙醇体积分数、乙醇用量、提取时间和提取次数对提取工艺的影响。采用HPLC测定大黄游离总蒽醌、栀子苷、咖啡酸含量

流动相分别为甲醇-0.1%磷酸溶液(85:15)

甲醇-水(23:77)

乙腈-0.1%磷酸溶液(10:90)

检测波长依次为254

238

323 nm。结果:最佳提取工艺参数为加12倍量80%乙醇提取3次

每次1 h。大黄游离总蒽醌、栀子苷、咖啡酸质量浓度分别为1 260.33

1 597.56

32.19 mg·L-1。结论:优选的痤疮消凝胶提取工艺合理可行、重复性好

为该制剂生产工艺参数的确定提供实验依据。

Abstract

Objective: To optimize extraction technique of Cuochuangxiao gel and provide a reference for clinical application of this preparation. Method: With composite score of contents of free anthraquinones in Rhei Radix et Rhizoma

gardenin and caffeic acid as index

uniform design was adopted to investigate effects of ethanol concentration

ethanol volume

extraction time and times on extraction process of Cuochuangxiao gel.HPLC was employed to determine contents of free anthraquinones in Rhei Radix et Rhizoma

gardenin and caffeic acid with mobile phases of methanol-0.1% phosphoric acid (85:15)

methanol-water (23:77) and acetonitrile-0.1% phosphoric acid (10:90)

detection wavelengthes of 254

238

323 nm

respectively. Result: Optimum extraction conditions were as follows:added 12 times the amount of 80% ethanol

extracted 3 times for 1 h each time.Under these conditions

contents of free anthraquinones in Rhei Radix et Rhizoma

gardenin and caffeic acid were 1 260.33

1 597.56

32.19 mg·L-1

respectively. Conclusion: This extraction process is reasonable

stable and feasible

which provides a theoretic basis for industrial production of Cuochuangxiao gel.

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Related Institution

Tian Jin Nan Kai Hospital

Zhejiang Chinese Medical University

Zhejiang Institute for Food and Drug Contrl

School of Chinese Materia Medica， Beijing University of Chinese Medicine

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