Objective: To investigate antioxidant activities and inhibitory effect on protein tyrosine phosphatase1B (PTP1B) of different fractions of Rubus idaeus

R.caesius and Ribes meyeri in Xinjiang

in order to screen out active ingredients with antioxidant activity and hypoglycemic action. Method: The different polar fractions were extracted by methanol and fractional extraction method. Then the total flavonoids content of different polar fractions were determined. The anthocyanin fractions of fruits were purified by AB-8macroporous resin. ABTS method

DPPH method and PTP1B inhibitory activity experiment were adopted to compare relevant activities of different fractions and analyze the correlation of flavonoid content and activity. Result: After AB-8macroporous resin purification

anthocyanin contents of R.idaeus and R.meyeri fruit extract increased from (12.7±0.14) mg·g-1 to (238.1±1.18) mg·g-1

and (35.3±0.45) mg·g-1 to (548.0±1.11) mg·g-1

respectively. With the increase of anthocyanin content

antioxidant activity and PTP1B inhibitory activity were also significantly increased. The ethyl acetate fraction of R.caesius stem had the highest total flavonoids content

which was (658.65±11.43) mg·g-1. The water fraction of R.caesiusstem had the highest antioxidant activity and PTP1B inhibitory activity

which was IC50(0.08±0.01) mg·L-1

and their ABTS and DPPH radical scavenging capacity were (4.53±0.07) and (2.82±0.09) mmol Trolox·g-1 equivalent

respectively. Conclusion: The different fractions of three plants have high flavonoid contents and good antioxidant activity and PTP1B inhibitory effect. The water fraction of R.caesius stem was the main active fraction. Anthocyanin of R.idaeus and R.meyeri fruits was the main active ingredient

and flavonoids from stem and leave of R.meyeri was the main antioxidant ingredient.